miRNA-9对骨肉瘤MG-63细胞增殖、凋亡的作用及其机制

目的：通过提高骨肉瘤MG-63细胞miRNA-9的表达，探讨miRNA-9对MG-63细胞增殖及凋亡的影响及可能的作用机制。方法：MG-63细胞随机分为实验组、阴性对照组和空白组。实验组转染miR-NA-9（ Oligo），阴性对照组转染阴性对照核苷酸序列（ microRNA negative control sequence），空白组不做转染。qRT-PCR检测细胞miRNA-9、CXCR4的表达。CCK-8法检测3组细胞的增殖；流式细胞术比较3组细胞的凋亡水平。结果：与阴性对照组和空白组相比，转染组细胞中miRNA-9表达升高；miRNA-9高表达的骨肉瘤细胞增殖速率降低、细胞凋亡率增加、CXCR4 mRNA转录水平下调，差异有统计学意义。结论：CXCR4基因参与miRNA-9抑制骨肉瘤MG-63细胞增殖过程，同时促进细胞凋亡。

The effects of microRNA-9 on proliferation and apoptosis of osteosarcoma MG-63 cells and correlated mechanisms

Objective:To study the function of miRNA-9 on osteosarcoma MG-63 cells proliferation and apop-tosis and correlated mechanisms by improving the expression of miRNA-9. Methods:Osteosarcoma MG-63 cells were randomly divided into three groups:Experimental group( transfected with miRNA -9 Oligo ),control group ( transfected with miRNA negative contrat)and blank group( transfected nothing). Detected the expression of miRNA-9,CXCR4 by real time PCR. Detected the CCK value(OD)at 24h,48h,72h and 96h,then observed the cell growth curve. The apoptosis was measured by flow cytometry. Results:Compared with negative control group and blank group,the miRNA transfection group was higher expressed on miRNA-9,osteosarcoma cells showed lower cell prolif-eration efficiency and higher cell apoptosis rotio on miRNA-9 higher expression groups. Conclusion:CXCR4 gene involved in the miRNA-9 inhition of osteosarcoma MG-63 cell proliferation,and promote cell apoptosis.