玉簪花的色谱鉴别和含量测定研究

目的:建立玉簪花的鉴别方法及其山柰素含量的测定方法。方法:鉴别采用薄层色谱法;含量测定采用高效液相色谱法,应用Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-0.4%磷酸溶液(60∶40)为流动相,流量为1 mL.min-1,检测波长为360 nm,柱温为30℃。结果:薄层色谱分离好,斑点清晰,阴性对照无干扰;山柰素在0.05～0.30μg范围内具有良好的线性关系,r=0.999 9,平均回收率为100.26%、RSD为1.69%(n=9)。结论:该方法灵敏、准确、专属性强,重现性好,可用于玉簪花的质量控制。

Study of Chromatography Identification and Content Determination of Hostae Flos

Objective： To establish a method for identification of Hostae Flos and determination of kaempferol in it. Methods： The TLC method was used to distinguish Hostae Flos and HPLC for determination of kaempferol. The Diamonsil Cis （4. 6 mm .250 mm,5 μm） column was used and methanol-0. 4% phosphoric acid solution（60： 40） as mobile phase with flow rate of 1 mL. min . The detection wavelength was 360 nm and column temperature was 30℃. Results ： The TLC identification was highly specific and spot of ofkaempferol was clear and there is no interference in negative comparison. Kaempferol showed a good linearity in a concentration range of 0. 05-0. 30 μg （r =0. 999 9） . The average recovery was 100. 26% and RSD was 1.69% （n =9）. Conclusion： This method was sensitive and accurate, in strong specificity and in good repeatability,it can be used for the quality control of Hostae Flos.