高效液相色谱法测定熊去氧胆酸片的含量 |
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引用本文: | 张燕,佟爱东.高效液相色谱法测定熊去氧胆酸片的含量[J].中国药品标准,2012,13(5):359-362. |
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作者姓名: | 张燕 佟爱东 |
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作者单位: | 广州市药品检验所,广州510160 |
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摘 要: | 目的:建立测定熊去氧胆酸片含量的方法。方法:色谱柱为Hypersil ODS2(4.6 mm×250 mm,5μm),流动相为0.001mol.L-1磷酸二氢钾溶液(磷酸调pH至2.0)–乙腈(50∶50),检测波长为210 nm,柱温40℃。结果:熊去氧胆酸进样量在1.8μg~104μg范围内线性良好(r=0.9998),平均回收率(n=9)为102.0%(RSD=1.2%)。结论:该方法准确、灵敏,重现性好,可有效控制熊去氧胆酸片的质量。
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关 键 词: | 熊去氧胆酸片 含量测定 高效液相色谱法 |
HPLC Determination of Content of Ursodeoxycholic Acid Tablets |
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Authors: | Zhang Yan and Tong Aidong |
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Institution: | ( Guangzhou Institute for Drug Control, Guangzhou 510160) |
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Abstract: | Objective: To establish an HPLC method for determination of the content of Ursodeoxycholic Acid Tablets. Method: A Hypersil ODS2 column (4. 6 mm x 250 ram,5μm)was used. Using the mobile phase of 0. 001mol L-1 potassium dihydrogen phosphate solution (adjust to pH 2.0 with phosphate acid acetonitrile (50: 50). The detection wavelength was set at 210nm and temperature was 40 ℃. Result: Ursodeoxyeholie acid showed a good linear relationship in the range of 1.8-104μg( r = 0. 999 8 ) , and the average recovery wasl02. 0% with RSD = 1.2% (n =9). Conclusion: The method is specific, accurate and convenient to be used for the quality control of Ursodeoxycholic Acid Tablets. |
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Keywords: | Ursodeoxycholic Acid Tablets content determination HPLC |
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