对《中国药典》2010年版苏合香丸鉴别方法改进的研究

目的:对《中国药典》2010年版一部苏合香丸的诃子、木香的薄层鉴别和麝香酮的GC鉴别方法进行改进。方法:以没食子酸对照品、诃子对照药材作对照,采用三氯甲烷-乙酸乙酯-甲酸(5∶4∶1)为展开剂进行鉴别;以木香对照药材作对照,采用石油醚(60～90℃)-乙酸乙酯(17∶3)为展开剂进行鉴别;以麝香酮作对照,采用柱长为2m,以聚乙二醇20000(PEG-20M)和5%二苯基-95%二甲基聚硅氧烷为混合固定相,涂布浓度分别为1.64%和1.32%,柱温为180℃进行GC鉴别。结果:采用改进后的薄层鉴别方法对诃子及木香进行鉴别,斑点更为清晰。采用改进后的GC鉴别方法对麝香酮进行鉴别,重现性较好,且能与样品中的各杂质峰实现良好分离。结论:改进后的薄层鉴别方法和GC鉴别方法准确、简便、专属性好。

Improvement on TLC and GC Identification for Shuhexiang Pills in Chinese Pharmacopoeia 2010 Edition

Objective：To improve the TLC identification for Chebulae Fructus and Aucklandiae Radix, and the GC identification formuscone. Methods： Gallic acid was chosen as chemical reference substance,and Chebulae Fructus as reference drag,and c.hloroform - ethyl acetate - formic acid （5：4：1 ）was chosen to he as developing agent to identify gdlic acid and Chebulae Fructus ; and using petroleum ether （ 60 - 90 ℃ ） - ethyl acetate （ 17： 3） as developing agent fi）r identification of Aucklandiae Radix; muscone as control, eolunm length is 2m, with polyethylene glycol 20 000 （PEG-20M） and 5% diphenyl - 95% dimethyl polysiloxane stationary phase as the mixed stationary phase, coating concentrations were 1.64% and 1.32% , the column temperature was 180 ℃ for GC identification. Result：Using the improved method, Chelmlae Fructus nd Aucklandiae Radix had more clear spots; GC identification of muscone was reliable and the sample peak achieved good separation with various impurities Conclusions：The improved TLC and GC identification methods are more accurate, simple with good specificity.