维生素C对大鼠急性肺损伤时铜、锌水平变化的影响

目的：了解大鼠急性肺损伤（ ALI）铜、锌水平的变化及维生素C对其的影响。方法将ALI实验动物随机分为13组。维生素C治疗组（ ALI后均立即给予维生素C治疗,依照采集标本不同时间点）分为：6 h组、8 h组、12 h组、16 h组和24 h组（均12 h追加一次维生素C）、36 h组（12和24 h各追加一次维生素C）,共6组;用生理盐水替换维生素C作为对照,共6组;阴性对照1组。用原子分光光度计检测肺组织铜、锌含量。结果铜：阴性对照组为0;治疗组为0;对照组6h、8h、12h 组呈直线下降趋势（组内差异明显,P ＜0.01）,16 h、24 h、36 h组为0。锌：治疗组和对照组组内均有明显差异（ P＜0.05）;各时间点治疗组与对照组相比均呈有意义改变（ P＜0.01）;各时间点的治疗组、对照组与阴性对照组相比均呈有意义改变（ P＜0.05或P＜0.01）。结论 ALI时铜有短暂升高;用VC后锌有一定程度升高,说明VC对ALI时锌的水平有一定调节作用。

The influence of vitamin C on the Copper and Zinc level of ALI rat

Objectove To understand the influence of vitamin C on the copper and zinc level in the ALI rats. Meth-ods The rats were divided into 13 groups,6 groups are administered vitamin C which samples were taken at different time point（6 h,8 h,12 h,16 h,24 h,36 h）,6 groups were control group while vitamin C were substituted by NS,and 1 group was set as negative control. The content of copper and zinc in the samples were detected by the atom spectro-photometer. ResuIts The Copper level in the negative control group,vitamin C group and 16 h,24 h,36 h control group were 0,and the copper level in the 6 h,8 h and 12 h control group show a downward trend. There were obvious differences in the zinc level between the vitamin C group and the control group（ P〈0 . 01 ）. concIusoon The vita-min C can regulate the zinc level when ALI in some extent.