| 哮喘大鼠肺T细胞中组蛋白去乙酰基酶1的表达及对组蛋白修饰的影响 |
| |
| 引用本文： | 李成业,郭雪君,甘丽杏,丁涛,顾问. 哮喘大鼠肺T细胞中组蛋白去乙酰基酶1的表达及对组蛋白修饰的影响[J]. 中国呼吸与危重监护杂志, 2010, 9(3): 259-263 |
| |
| 作者姓名： | 李成业 郭雪君 甘丽杏 丁涛 顾问 |
| |
| 作者单位： | 上海交通大学医学院附属新华医院呼吸科,上海,200092 |
| |
| 基金项目： | 国家自然科学基金，上海市教育委员会科研创新重点项目 |
| |
| 摘 要： | 目的明确哮喘大鼠肺T淋巴细胞中的组蛋白去乙酰基酶1(HDAC1)蛋白表达水平及组蛋白整体乙酰化和甲基化水平,探讨其在哮喘发病机制中的作用。方法 16只Wistar大鼠随机分为对照组和哮喘组(每组8只),用卵白蛋白(OVA)致敏并激发建立哮喘大鼠模型。通过哮喘临床表现、气道高反应性测定、肺组织HE染色、血清和BALF中细胞因子IL-4、γ干扰素(IFN-γ)及IgEELISA测定,判断哮喘大鼠模型是否成功。分离肺T细胞并进行纯度鉴定。Western blot检测肺T细胞HDAC1蛋白表达水平,组蛋白H3、H4整体乙酰化,以及H3k9整体二甲基化水平。结果与对照组比较,哮喘组HDAC1蛋白表达水平低于对照组(P0.05),组蛋白H3、H4整体乙酰化水平和H3k9整体二甲基化水平差异均无统计学意义。结论肺T细胞HDAC1可能参与了支气管哮喘的发病环节,组蛋白整体乙酰化和甲基化水平与哮喘发病无明显相关性,HDAC1对组蛋白修饰作用可能是一个基因转录特异性事件。
|
| 关 键 词： | 哮喘 T细胞 组蛋白去乙酰基酶1 组蛋白乙酰化 组蛋白甲基化 |
Expression of Histone Deacetylases 1 and It's Effects on Histone Modifications in T Cells Isolated from Lungs of Asthmatic Wistar Rats |
| |
| LI Cheng-ye,GUO Xue-jun,GAN Li-xing,DING Tao,GU Wen. Expression of Histone Deacetylases 1 and It's Effects on Histone Modifications in T Cells Isolated from Lungs of Asthmatic Wistar Rats[J]. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(3): 259-263 |
| |
| Authors: | LI Cheng-ye GUO Xue-jun GAN Li-xing DING Tao GU Wen |
| |
| Affiliation: | LI Cheng-ye,GUO Xue-jun,GAN Li-xing,DING Tao,GU Wen.Department of Respiratory Medicine,Xinhua Hospital,School of Medicine,Shanghai Jiaotong University.Shanghai,200092,China |
| |
| Abstract: | Objective To investigate the expression of the histone deacetylases 1(HDAC1) and the level of whole histone acetylation and methylation in lung T cells of asthmatic rats,and investigate their role in the pathogenesis of asthma.Methods Sixteen wistar rats were randomly divided into a control group and an asthma group(n=8 in each group).The rats was sensitized with ovalbumin(OVA) and challenged with aerosol OVA to establish asthma model.The asthmatic rat model was confirmed by measurement of pulmonary functio... |
| |
| Keywords: | Asthma T cells Histone deacetylases 1 Histone acetylation Histone methylation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
