DNA vaccine encoding myristoylated membrane protein (MMP) of rock bream iridovirus (RBIV) induces protective immunity in rock bream (Oplegnathus fasciatus) |
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| Affiliation: | 1. Department of Aqualife Medicine, Chonnam National University, Republic of Korea;2. Department of Marine Life Sciences, Jeju National University, Republic of Korea;1. Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, College of Life Sciences, Hubei University, Wuhan, Hubei 430062, PR China;2. Hubei Tianzhong Stockbreeding Co. Ltd., Wuhan, Hubei 430344, PR China;1. College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China;2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266000, China;1. Jeonju Biomaterials Institute, Jeonju 561-360, Republic of Korea;2. College of Veterinary Medicine, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea;1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;2. Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China;3. Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China |
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| Abstract: | Rock bream iridovirus (RBIV) causes severe mass mortalities in rock bream (Oplegnathus fasciatus) in Korea. In this study, we investigated the potential of viral membrane protein to induce antiviral status protecting rock bream against RBIV infection. We found that fish administered with ORF008L (myristoylated membrane protein, MMP) vaccine exhibited significantly higher levels of survival compared to ORF007L (major capsid protein, MCP). Moreover, ORF008L-based DNA vaccinated fish showed significant protection at 4 and 8 weeks post vaccination (wpv) than non-vaccinated fish after infected with RBIV (6.7 × 105) at 23 °C, with relative percent survival (RPS) of 73.36% and 46.72%, respectively. All of the survivors from the first RBIV infection were strongly protected (100% RPS) from re-infected with RBIV (1.1 × 107) at 100 dpi. In addition, the MMP (ORF008L)-based DNA vaccine significantly induced the gene expression of TLR3 (14.2-fold), MyD88 (11.6-fold), Mx (84.7-fold), ISG15 (8.7-fold), PKR (25.6-fold), MHC class I (13.3-fold), Fas (6.7-fold), Fas ligand (6.7-fold), caspase9 (17.0-fold) and caspase3 (15.3-fold) at 7 days post vaccination in the muscle (vaccine injection site). Our results showed the induction of immune responses and suggest the possibility of developing preventive measures against RBIV using myristoylated membrane protein-based DNA vaccine. |
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| Keywords: | Rock bream Rock bream iridovirus Protection DNA vaccine Myristoylated membrane protein Major capsid protein Immune responses |
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