Immune responses in bovines to recombinant glycoprotein D of bovine herpesvirus type 5 as vaccine antigen |
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| Affiliation: | 1. Laboratório de Bacteriologia, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas 96010-900, Brazil;2. Laboratório de Virologia e Imunologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul 96010-900, Brazil;1. Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul CEP 96160-000, Brazil;2. Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, Pará CEP 68740-970, Brazil;1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China;2. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China;1. The McConnell Group, Inc., Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, United States;2. U.S. Department of Homeland Security Science and Technology Directorate, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, United States;3. Oak Ridge Institute for Science and Education, Plum Island Animal Disease Center Research Participation Program, Oak Ridge, TN, United States;4. U.S. Department of Agriculture, Agricultural Research Service, Foreign Animal Disease Research Unit, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, United States;5. BioQuest Associates, LLC, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, United States;6. Benchmark Biolabs, Inc., 421 West Industrial Lake Dr., Lincoln, NE 68528, United States;7. Leidos, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, United States;8. GenVec, Inc., 910 Clopper Road, Suite 220N, Gaithersburg, MD 20878, United States |
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| Abstract: | Bovine herpesvirus 5 (BoHV-5) is responsible for outbreaks of meningoencephalitis that cause important economic losses in young cattle. BoHV-5 glycoprotein D (gD5) is essential for attachment and penetration into permissive cells and targeting of host immune systems, inducing strong humoral and cellular immune responses. The aim of this study was to evaluate the vaccinal immune response of vaccines formulated with the recombinant BoHV-5 gD (rgD5) in bovines. For the experiment, 72 heifers were randomly allotted into 6 different groups with 12 animals each. Group 1: vaccine formulated using inactivated BoHV-5 (iBoHV-5) adjuvanted with ISA50V2; Group 2: iBoHV-5 associated with 100 µg of rgD5 adjuvanted with ISA50V2; Group 3: 100 µg of rgD5 adjuvanted with ISA50V2; Group 4: 100 µg of rgD5 adjuvanted with Al(OH)3; Group 5: commercial vaccine; and Group 6: control group. Two doses were administered in a 26-day interval and the third after 357 days from primo vaccination. Cattle vaccinated with the vaccines formulated with iBoHV-5 plus rgD5 showed a significant (p < 0.01) five-fold increase in total immunoglobulin G (IgG) for BoHV-5, BoHV-1, and rgD5 as compared with the commercial and control groups. Also, a significant (p < 0.05) increase in IgG1 and IgG2a levels was induced in serum for rgD5. In addition, these same vaccines showed significant (p < 0.01) four-fold higher titers of BoHV-1 and -5 neutralizing antibodies. The results demonstrated that the rgD5 conserved important epitopes that were able to stimulate bovine humoral immunity response capable of viral neutralization of BoHV-1 and -5, suggesting it as a promising vaccine antigen to be used in vaccine for BoHV-1 and -5 endemic areas. |
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| Keywords: | Herpesvirus Adjuvant Subunit vaccine |
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