Rotavirus genotypes circulating in Ontario,Canada, before and after implementation of the rotavirus immunization program |
| |
Affiliation: | 1. Department of Paediatrics, The Hospital for Sick Children, Toronto, Ontario, Canada;2. Public Health Ontario, Toronto, Ontario, Canada;3. Département de géomatique, Université Laval, Québec, Québec, Canada;4. Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario, Canada;5. Department of Laboratory Medicine and Pathobiology and Department of Paediatrics, University of Toronto, Toronto, Ontario, Canada;1. Serviço de Endocrinologia, Diabetes e Metabolismo, Centro Hospitalar de São João, Porto, Portugal;2. Serviço de Endocrinologia, Diabetes e Metabolismo, Faculdade de Medicina da Universidade do Porto, Porto, Portugal;3. Serviço de Epidemiologia Clínica, Medicina Preditiva e Saúde Pública, Faculdade de Medicina da Universidade do Porto, Porto, Portugal;4. Serviço de Doenças Infeciosas, Centro Hospitalar de São João e Faculdade de Medicina da Universidade do Porto, Porto, Portugal;1. Divisão de Reumatologia, Departamento de Medicina, Hospital Raja Permaisuri Bainun Ipoh, Perak, Malásia;2. Departamento de Patologia, Hospital Raja Permaisuri Bainun Ipoh, Perak, Malásia |
| |
Abstract: | Background and objectivesOntario introduced a universal publicly-funded group A rotavirus (RVA) immunization program in August 2011, using monovalent vaccine. RVA immunization programs have decreased the incidence of RVA acute gastroenteritis in many countries but it is unclear if it will contribute to the emergence of certain genotypes. We monitored RVA trends and genotypes in Ontario before and after implementation of the publicly-funded immunization program.MethodsRVA detection was conducted at Public Health Ontario Laboratories from January 2009 to December 2011 (pre-program period) and January 2012 to October 2015 (publicly-funded RVA immunization program period) and number of RVA-positive specimens and percent positivity were analysed. A convenience sample of RVA-positive stool specimens, from September 2010 to December 2011 (pre-program period) and January 2012 to June 2013 (program period), were genotyped using heminested PCR. A literature review on the burden of illness from emergent genotype was performed.ResultsStool specimens showed a significant decrease in RVA percent positivity from the 36 month pre-program period (14.4%; 1537/10700) to the 46 month program period (6.1%; 548/9019). An increase in the proportion of RVA G10 among genotyped specimens, associated with five different P genotypes, from the pre-program (6.3%; 13/205) to the program (31.5%; 40/127) period was observed. Our literature review identified approximately 200 G10-positive human stool specimens from 16 different countries.ConclusionsThis study documented a decrease in the number of RVA-positive specimens and percent positivity after implementation of the immunization program. An unexpected increase in the proportion of RVA G10 was detected following program introduction. Ongoing RVA surveillance is important in evaluating both the long-term impact of immunization and emergence of RVA genotypes. |
| |
Keywords: | Rotavirus Immunization Genotype G10 Vaccine |
本文献已被 ScienceDirect 等数据库收录! |
|