Neutralization effects of antibody elicited by chimeric HBV S antigen viral-like particles presenting HCV neutralization epitopes |
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| Institution: | 1. Department of Clinical Laboratory and Research Center, Tangdu Hospital, The Fourth Military Medical University, No. 569 Xinsi Road, Xi''an, Shaanxi 710038, China;2. Department of Microbiology, The Fourth Military Medical University, No. 17 West Road, Xi''an, Shaanxi 710032, China;3. Department of Nephrology, Tangdu Hospital, The Fourth Military Medical University, No. 569 Xinsi Road, Xi''an, Shaanxi 710038, China;4. Department of Clinical Laboratory, Xi’an Third Hospital, No. 10 Eastern Section of The Third FengCheng Rd., WeiYang District, Xi’an, Shaanxi 710018, China;5. Department of Infectious Disease, Tangdu Hospital, The Fourth Military Medical University, No. 569 Xinsi Road, Xi''an, Shaanxi 710038, China;6. Laboratory Animal Research Center, The Fourth Military Medical University, No. 17 West Road, Xi''an, Shaanxi 710032, China;1. Fogarty International Center, National Institutes of Health, Bethesda, MD, USA;2. Viral Gastroenteritis Branch (proposed), Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA;1. Immunology Department, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, P.O. Box LG 581, Legon, Accra, Ghana;2. Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 209 l0-7500, USA;1. College of Life Sciences, Northeast Agricultural University, Harbin 150030, People''s Republic of China;2. The 211th Hospital of People''s Liberation Army, Harbin 150080, People''s Republic of China;3. Harbin Medical University, The Fourth Affiliated Hospital, Nangang District, Harbin 150001, People''s Republic of China;4. Harbin Pharmaceutical Group Bio-vaccine Co., Ltd., Harbin, 150000, People''s Republic of China;1. Department of Biochemistry, School of Life Sciences, Central University of Rajasthan, Bandarsindri, Kishangarh, Ajmer 305817, Rajasthan, India;2. Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India |
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| Abstract: | Hepatitis C virus (HCV) infection is a major public health problem despite effectual direct-acting antivirals (DAAs) therapy. Development of a prophylactic vaccine is essential to block spread of HCV infection. The HBV small surface antigen (HBsAg-S) can self-assemble into virus-like particles (VLPs), has higher immunogenicity and is used as a vaccine against HBV infections. Chimeric HBsAg-S proteins with foreign epitopes allow VLP formation and induce the specific humoral and cellular immune responses against the foreign proteins. In this study, we investigated the immune responses induced by chimeric VLPs with HCV neutralizing epitopes and HBV S antigen in mice. The chimeric HCV-HBV VLPs expressing neutralizing epitopes were prepared and purified. BALB/c mice were immunized with purified chimeric VLPs and the serum neutralizing antibodies were analyzed. We found that these chimeric VLPs induced neutralizing antibodies against HCV in mice. Additionally, the murine serum neutralized infections with HCV pseudoparticles and cell-cultured viruses derived from different heterologous 1a, 1b and 2a genotypes. We also found that immunization with chimeric VLPs induced anti-HBsAg antibodies. This study provides a novel strategy for development of a HCV prophylactic neutralizing epitope vaccine and a HCV-HBV bivalent prophylactic vaccine. |
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| Keywords: | Hepatitis C virus Epitopes Viral-like particles Neutralizing antibody Vaccine |
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