In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25 |
| |
| Institution: | 1. School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China;2. Key Lab of Biometallurgy of the Ministry of Education of China, Central South University, Changsha, 410083, China;3. CSIRO Mineral Resources, Clayton South, Vic, 3169, Australia;4. Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201204, China;5. School of Metallurgy, Northeastern University, Shenyang, 110819, China;1. School of Mining, College of Engineering, University of Tehran, Tehran 1439957131, Iran;2. Sarcheshmeh Copper Complex, Research and Development Centre, Rafsanjan 7731643181, Iran;3. Department of Materials Science and Metallurgy, Faculty of Chemical Sciences, Complutense University of Madrid, ES-28340 Madrid, Spain;4. Department of Microbiology, Ohio State University, Columbus, OH 43210, USA;1. Fundación Ciencia y Vida, Avenida Zañartu 1482, Ñuñoa, 7780272, Santiago, Chile;2. Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile;3. Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, WI, USA;4. Architecture et Fonction des Macromolécules Biologiques (AFMB), Aix-Marseille Univ-Centre National de la Recherche Scientifique (CNRS), UMR 7257, Campus de Luminy, Case 932, 13288, Marseille Cedex 09, France |
| |
| Abstract: | For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4). The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount. XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups ( C O, CO and CN). The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells. The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides. The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values. These differences are caused by the inconsistencies of measurement depth between XANES and the latter two characterization methods. |
| |
| Keywords: | Thermophile Bioleaching Extracellular polymeric substances Synchrotron radiation XANES |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
