Proliferative and osteogenic differentiation capacity of mesenchymal stromal cells: Influence of harvesting site and donor age |
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| Affiliation: | 1. Experimental Surgery and Regenerative Medicine (ExperiMed), Department of General, Trauma and Reconstructive Surgery, Munich University Hospital, Ludwig-Maximilians-University (LMU), Nussbaumstr. 20, 80336 Munich, Germany;2. Paracelsus Medical University (PMU) Salzburg, Strubergasse 21, 5020 Salzburg, Austria;1. Barts and The London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark St, Whitechapel, E1 2AT, London, UK;2. 401 General Military Hospital of Athens, P. Kanellopoulou Ave., Athens, 11525, Greece;3. 1st Propaedeutic Surgical Clinic, Athens Medical School, National and Kapodistrian University of Athens, 114 Vasilissis Sofias Avenue, Athens, 11527, Greece;4. 4th Surgical Clinic, "Attikon" University Hospital, National and Kapodistrian University of Athens, 1 Rimini str, Haidari, Athens, 12462, Greece;1. Harvard Combined Orthopaedic Residency Program, Boston, MA, USA;2. Beth Israel Deaconess Medical Center, Boston, MA, USA;1. Department Trauma TopCare, Elisabeth-TweeSteden Hospital, Tilburg, The Netherlands;2. Department of Public Health, Erasmus Medical Centre, Rotterdam, The Netherlands;3. Brabant Trauma Registry, Network Emergency Care Brabant, Tilburg, The Netherlands;4. Department of Surgery, Elisabeth-TweeSteden Hospital, Tilburg, The Netherlands;5. Department of Biomedical Data Sciences, Leiden University Medical Center, Leiden, The Netherlands;1. Department of General and Trauma Surgery, BG-University Hospital Bergmannsheil, Ruhr-University Bochum, Germany;2. Grand Rapids Medical Education Partners, Grand Rapids, MI, USA;3. Michigan State University, Grand Rapids, MI, USA;4. The CORE Institute®, Center for Orthopedic Research and Education, University of Arizona, College of Medicine, Phoenix, USA;5. Department of Trauma Surgery, University Hospital Essen, Germany;6. The CORE Institute®, Center for Orthopedic Research and Education, Banner Musculoskeletal Institute Center Chiefs for Orthopedic Trauma and Bone Health, University of Arizona, College of Medicine, Phoenix, USA;1. Ankara Training and Research Hospital, Department of Orthopaedics, Turkey;2. SBU Ankara Training and Research Hospital, Department of Pathology, Turkey |
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| Abstract: | Human mesenchymal stromal cells (hMSCs) are the cellular source of new bone formation and an essential component of autologous bone grafts. Autologous bone graft harvesting is routinely conducted at the iliac crest, although alternative donor sites with lower complication rates are available. Thus, the aim of this study was to compare hMSCs harvested from the iliac crest and the proximal tibia regarding their proliferative and osteogenic differentiation capacity. Furthermore, we investigated the influence of donor age on these biological properties.HMSCs were isolated from iliac crest or proximal tibia bone grafts of 46 patients. Proliferative capacity was assessed by cumulative population doublings, population doubling time, colony forming units and cell proliferation assays. Osteogenic capacity was assessed by quantification of extracellular calcium deposition and marker gene expression levels. The number of hMSCs per gram harvested tissue was determined. Furthermore, the adipogenic and chondrogenic differentiation capacity were quantified using BODIPY and Safranin Orange staining, respectively. Additional analyses were carried out after grouping young (18–49 years) and aged (≥50 years) donors.HMSCs derived from the proximal tibia featured a comparable proliferative and osteogenic differentiation capacity. No significant differences were found for any analysis conducted, when compared to hMSCs obtained from the iliac crest. Furthermore, no significant differences could be revealed when comparing young and aged donors. This was equally true for hMSCs from both donor sites after comparison within the same age group.Our study demonstrates comparable biological properties of hMSCs derived from both donor sites, the iliac crest and the proximal tibia. Furthermore, aging does not alter proliferative and osteogenic differentiation capacity. Consequently, the proximal tibia should be considered more closely as an alternative donor site in patients of all age groups. |
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| Keywords: | Human mesenchymal stromal cell Proliferation Osteogenic differentiation Donor site Autologous bone graft Iliac crest Proximal tibia |
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