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Effects on G2/M Phase Cell Cycle Distribution and Aneuploidy Formation of Exposure to a 60 Hz Electromagnetic Field in Combination with Ionizing Radiation or Hydrogen Peroxide in L132 Nontumorigenic Human Lung Epithelial Cells
Authors:Hee Jin  Hye Eun Yoon  Jae-Seon Lee  Jae-Kyung Kim  Sung Ho Myung  Yun-Sil Lee
Affiliation:1.Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Korea.;2.Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 402-751, Korea.;3.Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185, Korea.;4.Power Systems Research Division, Electrical Environment Team, Korea Electrotechnology Research Institute, Changwon 642-120, Korea.
Abstract:The aim of the present study was to assess whether exposure to the combination of an extremely low frequency magnetic field (ELF-MF; 60 Hz, 1 mT or 2 mT) with a stress factor, such as ionizing radiation (IR) or H2O2, results in genomic instability in non-tumorigenic human lung epithelial L132 cells. To this end, the percentages of G2/M-arrested cells and aneuploid cells were examined. Exposure to 0.5 Gy IR or 0.05 mM H2O2 for 9 h resulted in the highest levels of aneuploidy; however, no cells were observed in the subG1 phase, which indicated the absence of apoptotic cell death. Exposure to an ELF-MF alone (1 mT or 2 mT) did not affect the percentages of G2/M-arrested cells, aneuploid cells, or the populations of cells in the subG1 phase. Moreover, when cells were exposed to a 1 mT or 2 mT ELF-MF in combination with IR (0.5 Gy) or H2O2 (0.05 mM), the ELF-MF did not further increase the percentages of G2/M-arrested cells or aneuploid cells. These results suggest that ELF-MFs alone do not induce either G2/M arrest or aneuploidy, even when administered in combination with different stressors.
Keywords:Aneuploidy   Extremely low frequency   G2/M phase   H2O2   Ionizing radiation
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