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| 免疫印迹法检测肝纤维化组织基质金属蛋白酶抑制因子-1表达水平 | |
| 引用本文： | 黄宇琦,李朝龙,魏银燕,高毅. 免疫印迹法检测肝纤维化组织基质金属蛋白酶抑制因子-1表达水平[J]. 广东医学, 2001, 22(7): 569-570 |
| 作者姓名： | 黄宇琦 李朝龙 魏银燕 高毅 |
| 作者单位： | 1. 第一军医大学南方医院肝胆外科, 2. 第一军医大学南方医院校办, 3. 珠江医院肝胆外科, |
| 基金项目： | 广东省自然科学基金资助课题 (编号 :960 362 ) |
| 摘 要： | 目的 观察实验性肝纤维化过程中金属蛋白酶组织抑制因子－1（TIMP－1）蛋白表达水平的动态变化。方法 建立四氯化碳中毒性大鼠肝纤维化模型，采用免疫印迹方法（Western blot法），测定肝纤维化不同阶段TIMP－1的蛋白表达水平。结果 正常大鼠肝组织可见TIMP－1的蛋白表达。在肝纤维化的发生、发展过程中，TIMP－1蛋白表达逐渐增强，到肝硬化阶段达到高峰。结论 TIMP－1可能是促进和影响肝纤维化、肝硬化形成的重要因素。 |
| 关 键 词： | 金属蛋白酶 组织抑制因子－1 蛋白表达 实验性 肝纤维化 ECM MMPS 免疫印迹法 |
| 修稿时间： | 2001-03-06 |
Tissue inhibitor of metalloproteinase-1 protein expression is enhanced in experimental liver fibrosis |
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| Huang Yuqi,Wei Yinyan,Gao Yi,et al.. Tissue inhibitor of metalloproteinase-1 protein expression is enhanced in experimental liver fibrosis[J]. Guangdong Medical Journal, 2001, 22(7): 569-570 | |
| Authors: | Huang Yuqi Wei Yinyan Gao Yi et al. |
| Affiliation: | Huang Yuqi,Wei Yinyan,Gao Yi,et al. Department of Hepato biliary Surgery,Nanfang Hospital,First Military Medical University,Guangzhou 510515 |
| Abstract: | To investigate the dynamic alteration of tissue inhibitor of metalloproteinase 1(TIMP 1) protein expression in experimental liver fibrosis. Methods CCl 4 induced animal models of liver fibrosis was established and TIMP 1 protein expression level in different stages of liver fibrosis was measured by Western blot. Results TIMP 1 protein expression was detected in normal rat liver.TIMP 1 protein expression level was increased gradually in the development of liver fibrosis, reached its peak at the stage of liver cirrhosis. Conclusion TIMP 1 may be an important factor in promoting liver fibrosis and cirhosis. |
| Keywords: | Tissue inhibitor of metalloproteinase 1 Protein expression Experimental liver fibrosis. |
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