某市食管癌组织中人类乳头状瘤病毒感染情况的研究

目的研究河南省食管癌高发区食管癌组织中人类乳头状瘤病毒感染与食管鳞状细胞癌发生的关系。方法利用PCR和免疫组织化学技术检测68例食管癌，15例非典型增生食管上皮和53例正常食管黏膜组织中人类乳头状瘤病毒HPV16／18感染和HPV16／18E6蛋白的表达情况。结果68例食管癌中，HPV DNA阳性率为67．6％（46／68）。在正常食管黏膜、非典型增生上皮及鳞状细胞癌组织中HPV16／18E6的阳性率分别为9．4％（5／53）、40．0％（6／15）和61．2％（42／68），癌组织中HPV16／18E6蛋白的阳性表达率明显高于非典型增生上皮和正常食管黏膜（P〈0．05），非典型增生上皮中HPV16／18E6蛋白的阳性表达率亦明显高于正常食管黏膜（P〈0．05）。结论HPV16／18型感染可能在某食管癌高发区食管癌的发生中起重要作用。

Study of Human Papilloma Virus on Esophageal Carcinoma Tissue in one County

Objective To investigate the relation of human papilloma virus(HPV) of esophageal carcinoma tissue and esophageal squamous cell carcinogenesis in one county. Methods Immunohistochemistry and PCR were used to detect the infection of HPV 16/18 and the expression of HPV 16/18 E6 protein in esophageal squamous cell carcinomas(68 cases),dysplasia tissues(15 cases) and normal esophageal mucosa tissues(53 cases). Results The positive rate of HPV DNA in esophageal carcinoma(68 cases) was 67.6%(46/68).The positive rates of HPV 16/18 E6 protein in normal esophageal mucosa tissues,dysplasia tissues and esophageal squamous cell carcinomas were 9.4%(5/53),40.0%(6/15) and(61.2)%(42/68) respectively.The positive rate of HPV 16/18 E6 protein in cancer tissues was significantly higher than those in dysplasia tissues and normal esophageal mucosa tissues(P<0.05). The positive rate of HPV 16/18 E6 protein in dysplasia tissues was significantly higher than that in normal esophageal mucosa tissues(P<0.05). Conclusion The data suggest that HPV 16/18 infection may play an important role in the development of esophageal carcinomas in the high incidence region.