体外构建色素化皮肤类似物模型

目的 探寻构建色素化皮肤类似物的方法.方法 以包皮组织作为细胞来源,采用消化法获得角质形成细胞、黑素细胞;采用组织块法获得成纤维细胞,以鼠尾胶原为支架,构建色素化皮肤类似物.HE染色、Fontana Masson染色检测构建的色素化皮肤类似物的大体结构及其中黑色素细胞的分布和状态,以透射电镜观察类似物的超微结构.结果 构建的色素化皮肤类似物结构完整,细胞状态良好,与皮肤结构相似.结论 成功构建了色素化皮肤类似物模型.

Construction of an in vitro equivalent of the pigmented skin

Objective To construct an in vitro equivalent of the pigmented skin using tissue engineering methods.Methods Surgically removed foreskins was used as the source of keratinocytes and melanocytes harvested by routine tissue digestion.The fibroblasts were enriched by tissue block culture and seeded into the scaffold constructed using mouse tail collagens to construct the pigmented skin equivalent model.The general structure and the melanocyte distribution and growth status in this model were observed with HE staining and Fontana Masson staining.The ultrasctructure of the constructed pigmented skin equivalent was observed by transmission electron microscope.Results and Conclusion The pigmented skin equivalent model was structurally intact,and allowed optimal cell growth.Fontana Masson staining identified in the basal layer numerous melanocytes in normal growth,and the constructed model was structurally similar to normal skin tissue,suggesting successful construction of the pigmented skin equivalent model.