蜂毒肽对人神经胶质瘤细胞的增殖抑制和诱导凋亡作用

目的 研究蜂毒肽对体外培养的入神经胶质瘤细胞的增殖抑制和诱导凋亡的作用.方法 MTT法检测蜂毒肽对两种人神经胶质瘤细胞(U87、U251)的增殖抑制率;流式细胞仪和凝胶电泳法检测蜂毒肽对两种肿瘤细胞的凋亡诱导作用.结果 蜂毒肽能够明显抑制两种细胞的生长(P＜0.05);浓度为1、10、20、40、80、160、200 mg/L的蜂毒肽诱导U87和U251细胞的凋亡率分别达到12.80%、16.92%、22.69%、34.05%、41.82%、59.87%、80.25%和11.61%、16.21%、22.03%、30.57%、41.10%、58.33%、79.12%,其诱导凋亡作用与剂量呈正相关.结论 蜂毒肽对人神经胶质瘤细胞具有明显的增殖抑制和促进凋亡作用.

Melittin inhibits proliferation and induces apoptosis of malignant human glioma cells

Objective To investigate the anti-tumor effects of melittin against malignant human glioma cells in vitro.Methods Two malignant human glioma cell lines(U87 and U251) were treated with melittin at various concentrations,and the cell growth inhibition and apoptosis were evaluated using MTT assay,flow cytometry and agarose gel electrophoresis.Results Melittin could obviously inhibit the proliferation of the two glioma cell lines(P<0.05).At the concentrations of 1,10,20,40,80,160,200 mg/L,melittin resulted in U87 cell apoptosis rates of 12.80%,16.92%,22.69%,34.05%,41.82%,59.87%,and 80.25%,and in U251 cell apoptosis rate of 11.61%,16.21%,22.03%,30.57%,41.10%,58.33%,and 79.12%,respectively,showing a dose-dependent effect in its action of inducing cell apoptosis.Conclusion Melittin inhibits the proliferation and induces apoptosis of malignant human glioma cell lines in vitro.