VEGF和metastin在大肠癌组织中的表达

目的 研究血管内皮生长因子(VEGF)和KISS-1基因编码产物metastin在大肠癌组织中的表达情况及其与大肠癌临床病理因素特征之间的关系.方法 应用免疫组化SP法检测70例大肠癌组织和癌周正常组织中VEGF和metastin的表达水平.结果 VEGF在大肠癌组织中的阳性表达率为48.6%(34/70),在癌旁正常组织中的阳性表达率为0.00%(0/70),两者比较有显著性差异(P＜0.01);metastin在大肠癌组织中的阳性表达率为28.6%(20/70),在癌旁正常组织中的阳性表达率为70.0%(49/70),两者比较有显著性差异(P＜0.01).VEGF表达水平与大肠癌组织学分化程度、浸润深度、TNM分期、淋巴结转移有关(P＜0.01),metastin表达水平与大肠癌浸润深度、TNM分期、淋巴结转移有关(P＜0.05).结论 同时检测大肠癌组织中VEGF与metastin表达水平,对判断大肠癌恶性程度和预后具有重要价值.

Expression of vascular endothelial growth factor and metastin in colorectal carcinoma

Objective To study the expression of vascular endothelial growth factor(VEGF)and metastin in colorectal carcinoma and their association with the clinicopathological features of the malignancy.Methods VEGF and metastin expressions were examined immunohistochemically with SP method in 70 specimens of human colorectal carcinoma tissues and adjacent normal tissues.Results VEGF protein overexpression was detected in 48.6%(34/70)of the colorectal carcinoma tissues but in none of the adjacent normal tissues(P<0.01),and for metastin,the overexpression rate was 28.6%(20/70)in the colorectal carcinoma tissues and 70.0%(49/70)in the normal tissues(P<0.01).The expression of both VEGF and metastin was related to the histological grades,infiltration depth,TNM stage and lymph node metastasis of the tumor(P<0.01 and P<0.05,respectively).Conclusion Immunohistochemical detection of VEGF and metastin can be of value in assessment of the malignancy and in prognostic evaluation of colorectal carcinoma.