支链氨基酸肠外营养对肝硬化大鼠肝部分切除术后蛋白质代谢的影响

目的 探讨富含支链氨基酸的肠外营养对肝硬化大鼠肝部分切除术后蛋白质代谢的影响.方法 6只正常大鼠作为正常对照组,18只肝硬化大鼠随机分为肝硬化术前组(6只)、肝部分切除术后行8.5%Novamin肠外营养5 d组(6只)和肝部分切除术后行10%Hepa肠外营养5 d组(6只).测大鼠血清白蛋白(ALB)、胰岛素样生长因子-1(IGF-1)水平和血浆氨基酸谱,用RT-PCR法检测肝组织ALB mRNA表达.结果 与8.5%Novamin肠外营养比较,10%Hepa肠外营养5 d后血清ALB无明显差别,血清IGF-1水平、Fischer值和肝组织ALB mRNA表达水平明显升高(P＜0.05).结论 富含支链氨基酸的肠外营养可以改善肝硬化大鼠肝部分切除术后血浆氨基酸谱的构成,促进蛋白质合成.

Effect of branched-chain amino acid-rich parenteral nutrition on protein metabolism after partial hepatectomy in rats with liver cirrhosis

Objective To observe the effect of parenteral nutrition(PN) with branched-chain amino acid supplementation on protein metabolism after partial hepatectomy in rats with liver cirrhosis.Methods Eighteen rats with liver cirrhosis were randomly divided into pre-operation group(n=6),post-operation 8.5% Novamin PN group(n=6) and post-operation 10% Hepa PN group(n=6),with 6 normal rats severing as the normal control group.Five days after the operation,serum albumin(ALB),insulin-like growth factor I(IGF-1) and plasma amino acid spectrum were measured,and ALB mRNA level in the liver was assayed using RT-PCR.Results Postoperative serum ALB was similar between 10% Hepa PN and 8.5% Novamine PN groups,but the rats in the latter group showed significantly increased serum IGF-1 level,Fischer ratio and hepatic ALB mRNA expression(P<0.05).Conclusion Administration of PN with branched-chain amino acid supplementation can ameliorate plasma amino acid spectrum and increase protein synthesis in rats with liver cirrhosis after partial hepatectomy.