宫颈癌和子宫内膜癌组织中LIV-1基因mRNA的表达及意义

目的 探讨雌激素相关基因LIV-1 mRNA在宫颈癌和子宫内膜癌组织中的表达及其意义.方法 采用逆转录PCR方法检测49例宫颈癌、11例正常宫颈组织、24例子宫内膜癌和5例正常子宫内膜中LIV-1基因的mRNA水平,并分析其与各临床病理指标的相关性.结果 LIV-1 mRNA在正常宫颈组织、宫颈原位癌和宫颈浸润癌中的表达水平分别为0.20±0.16、0.31±0.13和0.40±0.15,差别具有统计学意义(方差分析P=0.000);但LIV-1 mRNA水平在不同FIGO分期、病理分级、浸润深度和淋巴结转移之间无显著变化(P均＞0.05).子宫内膜癌和正常子宫内膜LIV-1 mRNA水平的差异无统计学意义(分别为0.62±0.22和0.65±0.18,P＞0.05).结论 LIV-1基因的表达上调具有组织特异性,其可能参与了宫颈癌的发生发展,而与子宫内膜癌的进展无关.

Expression of LIV-1 mRNA in human cervical carcinoma and endometrial carcinoma

OBJECTIVE: To investigate the mRNA expression of oestrogen-regulated gene LIV-1 in human cervical carcinoma and endometrial carcinoma. METHODS: RT-PCR was performed to detect the expression of LIV-1 mRNA in 49 cervical carcinoma, 11 normal cervical tissue, 24 endometrial carcinoma and 5 normal endometrial specimens. RESULTS: The relative quantity of LIV-1 mRNA in normal cervical tissue, cervical carcinoma in situ and cervical carcinoma was 0.20-/+0.16, 0.31-/+0.13 and 0.40-/+0.15, respectively (P<0.05). No significant variation in LIV-1 mRNA expression was noted in relation to FIGO stages, histological differentiation, invasion depth and lymph node metastasis of the tumor (P>0.05), and LIV-1 mRNA level was similar between endometrial carcinoma and normal endometrial tissues (P>0.05). CONCLUSION: LIV-1 mRNA upregulation seems to be tissue-specific, and LIV-1 can be associated with the progression of cervical squamous cell carcinoma progression but not with the development of endometrial carcinoma.