| 气相色谱法测定血浆中丙戊酸浓度的方法改进 |
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| 引用本文： | 马虹英,李新中. 气相色谱法测定血浆中丙戊酸浓度的方法改进[J]. 中国药房, 2002, 13(9): 544-545 |
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| 作者姓名： | 马虹英 李新中 |
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| 作者单位： | 中南大学附属湘雅医院药剂科,长沙市410008 |
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| 摘 要： | 目的 :建立大口径毛细管气相色谱法测定人体血浆中丙戊酸浓度。方法 :FFAP0 53mm×30mID为色谱柱 ,柱温为180℃ ,N2 为载气 ,FID为检测器 ,联苯为内标 ,血浆样品加硫酸酸化 ,经氯仿萃取后直接进样。结果 :内标法和外标法的线性范围为25 3～303 6μg/ml,方法回收率均在96 0 %～100 7 %之间 ,两种方法无显著性差异 (P<0 05)。结论 :可采用外标法代替内标法定量。
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| 关 键 词： | 气相色谱法 丙戊酸 血药浓度 |
| 文章编号： | 1001-0408(2002)09-0544-02 |
| 修稿时间： | 2002-02-19 |
Rapid Determination of Valproate in Plasma by Large- bore Capillary Gas Chromatography |
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| MA Hongying,LI Xinzhong. Rapid Determination of Valproate in Plasma by Large- bore Capillary Gas Chromatography[J]. China Pharmacy, 2002, 13(9): 544-545 |
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| Authors: | MA Hongying LI Xinzhong |
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| Abstract: | OBJECTIVE:A large-bore capillary gas chromatography method has been developed to determine the concentration of valproate in human plasma METHODS:A FFAP phase 0 53mm×30m ID capillary column was used,the column temperature was 180℃ The carrier gas was nitrogen The detector was FID The internal standard was diphenyl The plasma was extracted with chloroform after adding sulfuric acid The extract was injected directly RESULTS:The external standard method and the internal standard method showed a good linearity from 25 3～303 6μg/ml The recoveries were 96 0%～100 7% for valproate There was no significant difference between the two methods(P>0 05) CONCLUSION:It is advisable to use the external standard method to determine the concentration of valproate in human plasma |
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| Keywords: | gas chromatography valproate blood drug concentration |
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