| 人AQP1基因的重组腺病毒构建及鉴定 |
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| 引用本文： | 杨彦春,杨军,周继祥,李慧增,肖林.人AQP1基因的重组腺病毒构建及鉴定[J].第三军医大学学报,2004,26(6):530-532. |
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| 作者姓名： | 杨彦春 杨军 周继祥 李慧增 肖林 |
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| 作者单位： | 第三军医大学西南医院口腔科,重庆,400038;第三军医大学西南医院口腔科,重庆,400038;第三军医大学西南医院口腔科,重庆,400038;第三军医大学西南医院口腔科,重庆,400038;第三军医大学西南医院口腔科,重庆,400038 |
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| 摘 要： | 目的构建人AQP1(hAQP1)基因的重组腺病毒载体.方法采用DNA重组与细菌内同源重组技术,构建含hAQP1的复制缺陷型重组腺病毒载体(pAdEasy-1/ hAQP1),并观察pAdEasy-1/ hAQP1在ECV-304中的感染情况.PCR方法鉴定重组腺病毒载体,利用绿色荧光蛋白GFP检测病毒滴度和感染效率.结果 PCR及限制性内切酶酶切鉴定证明pAdEasy-1/ hAQP1构建成功.结论应用细菌内同源重组法成功构建了含hAQP1基因的重组腺病毒载体.
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| 关 键 词： | 腺病毒 AQP1 同源重组 |
| 文章编号： | 1000-5404(2004)06-0530-03 |
| 修稿时间： | 2003年10月15 |
Construction and identification of human aquaporin 1 gene recombinant adenovirus |
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| YANG Yan chun,YANG Jun,ZHOU Ji xiang,LI Hui zeng,XIAO Lin.Construction and identification of human aquaporin 1 gene recombinant adenovirus[J].Acta Academiae Medicinae Militaris Tertiae,2004,26(6):530-532. |
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| Authors: | YANG Yan chun YANG Jun ZHOU Ji xiang LI Hui zeng XIAO Lin |
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| Abstract: | Objective To construct the recombinant adenovirus of human aquaporin 1 (AQP1) gene. Methods The replication deficient recombinant adenovirus encoding hAQP 1(pAdEasy 1/ hAQP1) was constructed by the method of DNA recombination and homogenous recombination in bacteria. The ECV 304 cells were transfected by the recombinant adenovirus. The target gene was identified by polymerase chain reaction (PCR). The titer and the infection rate were determined using the green fluorescent protein (GFP) expression in the shuttle plasmid. Results Restriction endonuclease and PCR analyses confirmed that the recombinant adenovirus was successfully constructed. The infection rate was high. Conclusion The recombinant adenovirus pAdEasy 1/ hAQP1 has been successfully constructed by the method of homogenous recombination in bacteria. |
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| Keywords: | AQP1 |
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