地塞米松对骨髓基质细胞生物学特性的影响

目的 探讨地塞米松对体外培养的骨髓基质细胞增殖和分化等生物学特性的影响。方法 取4－6周龄新西兰兔双侧股骨骨髓3ml，体外分离，培养至第3代骨髓基质细胞，分别用地塞松浓度为0、10^10、10^-9、10^-8、10^-7和10^-6mol/L的培养基，作用2、4、及6天后，测定骨髓基质细胞的分裂增殖能力和碱性磷酸酶活性。结果 地塞米松对骨髓基质细胞的增殖起抑制作用，并随地塞米松浓度的升高而增强，其浓度大于10^-8mol/L后抑制作用越显著。地塞米松在抑制增殖的同时，可显著增强骨髓基质细胞的碱性磷酸酶活性，浓度越高作用也越显著，但超过10^-8mol/L后各浓度的作用效果无显著差别；这种作用随时间的延长而增强，至6天时与对照组相比可增强2－4倍。结论 地塞米松抑制骨髓基质细胞的增殖，但可促进其分化成骨细胞， 浓度为10^-8mol/L较合适。

THE EFFECTS OF DEXAMETHASONE ON BIOLOGICAL CHARACTERISTICS OF BONE MARROW STROMAL CELLS

Objective To investigate the effects of dexamethasone on the proliferation and differentiation of bone marrow stromal cells(MSC). Methods MSC were isolated and cultured in vitro. After treatment with different concentrations of dexamethasone (0, 10 10 , 10 9 , 10 8 , 10 7 and 10 6 mol/L), the proliferation and alkaline phosphatase (ALP) activity of MSC were measured to evaluate the effect of dexamethasone on the biological characteristics of MSC. Results Dexamethasone inhibited cell proliferation. With the increase of concentration of dexamethasone, the effect was enhanced, which was more significant when the concentration of dexamethasone was over 10 8 mol/L. At the same time, dexamethasone promoted the activity of ALP. This effect was enhanced with the increase of concentration of dexamethasone, but the alteration was small when the concentration of dexamethasone was over 10 8 mol/L. The effects increased with the time. The activity of ALP was enhanced 2 to 4 times with the dexamethasone for 6 days. Conclusion Dexamethasone inhabit the proliferation of MSC, while induce them to differentiate into osteoblasts. The appropriate concentration of dexamethasone was 10 -8 mol/L.