A Study on the Mechanism of Bruceine D in the Treatment of Non‑small Cell Lung Cancer H1299 Cells |
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作者姓名: | Xiao‑Yun Shen Chao‑Yue Su Yan‑Yan Yan Ling‑Ling Zhang Qiao‑Ru Guo Hu‑Biao Chen Shuhela Zhumabieke Yelxat Danabek Jia‑Jun Li Yun Liu Bolat Makabel Jian‑Ye Zhang |
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作者单位: | a.Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, P. R. China;b.Xinjiang Institute of Materia Medica, Urumqi, P. R. China;c.Institute of Traditional Chinese Medicine,
Xinjiang Medical University, Urumqi, P. R. China;a.Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, P. R. China;d.Collaborative Innovation Center for Cancer, Institute of Respiratory and Occupational Diseases, Medical College, Shanxi Datong University, Datong, P. R. China;e.School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, P. R. China;b.Xinjiang Institute of Materia Medica, Urumqi, P. R. China;c.Institute of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi, P. R. China;f.Key Laboratory Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, P. R. China |
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摘 要: | Background: Non?small cell lung cancer (NSCLC) is considered one of the leading causes of cancer?related death. Despite the availability of drugsfor the treatment of NSCLC, the need for the development of novel agents with high efficiency and fewer adverse effectsremains unmet. The natural compound bruceine D (BD) is widely recognized for its notable anti?inflammatory, antiparasitic, and hypoglycemic activities. However, it is unclear whether BD can be used as a novel agent for NSCLC treatment. Materials and Methods: MTT and colony formation assays were used to assess the antiproliferative effect of BD on NSCLC cells. Wound healing and transwell assays were performed to determine the effect of BD on the migration and invasion of H1299 cells, respectively. Western blotting assay was used to detect the expression levels of proteins. Results:We demonstrated thatBD significantly inhibited the proliferation of H1299,A549, and H226 cells with respective IC50 values of 6.06 ± 0.52, 7.15 ± 0.90, and 7.21 ± 0.75 μM. In addition, BD suppressed colony formation of H1299 cells in a dose?dependent manner. Following treatment with BD, the migration and invasive capabilities of H1299 cells were significantly inhibited in a dose? and time?dependent manner. Moreover, the results of Western blotting demonstrated that BD treatment resulted in the upregulation of the protein expression of E?cadherin and downregulation of the expression of N?cadherin, twist, snail, integrin αv, integrin β4, matrix metalloproteinase-7, and β-catenin proteins. Conclusion: BD inhibits proliferation, migration, and invasion of NSCLC cells; therefore, BD may be considered for its potential in adjuvant therapy for NSCLC.
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关 键 词: | Bruceine D invasion migration non?small cell lung cancer proliferation |
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