| 微波解冻法对冷冻组织细胞角蛋白免疫组化检测影响的实验研究 |
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| 引用本文： | 黎相照,李林,徐小艳,申洪.微波解冻法对冷冻组织细胞角蛋白免疫组化检测影响的实验研究[J].中国医学物理学杂志,2011,28(5):2941-2944. |
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| 作者姓名： | 黎相照 李林 徐小艳 申洪 |
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| 作者单位： | 1. 南方医科大学南方医院病理科,广东广州510515;南方医科大学基础医学院病理系,广东广州510515
2. 南方医科大学南方医院病理科,广东广州,510515 |
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| 摘 要： | 目的：揭示微波解冻对冻结组织免疫组化染色效果的影响,为用微波解冻冷冻组织进行免疫组化染色提供实验依据。方法：将清洁级大耳白家兔分为常规对照组、自然解冻组、温水解冻组、微波解冻组。用空气将家兔栓塞致死后取其肝、肺、肾组织,包埋并制成切片,进行广谱细胞角蛋白免疫组化染色并观察染色结果。结果：微波解冻对肝、肾、肺冻结组织中细胞角蛋白抗原的影响不明显,均能正常显色。结论：冻结组织微波解冻对细胞角蛋白的免疫组化检查未见明显不良影响,提示温和的微波解冻对组织中细胞角蛋白抗原影响不明显。
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| 关 键 词： | 微波解冻 免疫组织化学 细胞角蛋白 |
Effects of Microwave Thawing to Frozen Tissue on Cytokeratin Stained by Immunohistochemistry |
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| LI Xiang-zhao,LI lin,XU Xiao-ya,SHEN Hong.Effects of Microwave Thawing to Frozen Tissue on Cytokeratin Stained by Immunohistochemistry[J].Chinese Journal of Medical Physics,2011,28(5):2941-2944. |
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| Authors: | LI Xiang-zhao LI lin XU Xiao-ya SHEN Hong |
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| Institution: | LI Xiang-zhao1,2,LI lin1,XU Xiao-yan1,SHEN Hong1 (1.Department of Pathology,Nangfang Hosiptal,Southern Medical University,Guangzhou Guangdong 510515,China,2.Department of Pathology,School of Basic Medical Sciences,China) |
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| Abstract: | Objective: Using immunohistochemistry methods to detect the affection of microwave thawing to frozen tissue on CK.Methods : The clean-level big-ear white rabbits were divided into 4 group,normal,natural thawing,warm water thawing and microwave thawing group.Rabbits were killed by air embolism.After which the liver,lung and kidney tissue sections were embedded,sliced,HE stained,and immunohistochemical stained with cytokeratin(CK).Results: The affection of microwave thawing to frozen tissue on CK antigen was ... |
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| Keywords: | microwave thawing immunohistochemistry cytokeratin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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