| AD5-10与表阿霉素联合作用治疗类风湿性关节炎 |
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| 引用本文： | Zhou JS,Shi J,Zhu JQ,Yuan HQ,Liu YX,You X,Zheng DX. AD5-10与表阿霉素联合作用治疗类风湿性关节炎[J]. 中国医学科学院学报, 2011, 33(4): 367-370. DOI: 10.3881/j.issn.1000-503X.2011.04.004 |
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| 作者姓名： | Zhou JS Shi J Zhu JQ Yuan HQ Liu YX You X Zheng DX |
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| 作者单位： | 中国医学科学院北京协和医学院基础医学研究所医学分子生物学国家重点实验室,北京,100005 |
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| 基金项目： | 国家自然科学基金,院所长基金 |
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| 摘 要： | 目的 研究抗死亡受体抗体(AD5-10)与表阿霉素联合作用治疗类风湿性关节炎的机制.方法 采用MTI法检测成纤维样滑膜细胞(FLS)存活率,用蛋白免疫印迹研究细胞凋亡信号通路蛋白和p53、p21的变化.结果 不同剂量的表阿霉素可增强FLS对AD5-10的敏感性,促进FLS凋亡.表阿霉素处理可使FLS细胞中caspase-3、-8、-9,c-FLIP,Bc1-2,p53和p21表达水平发生变化.结论 表阿霉素可能通过改变p53、p21、c-FLIP和Bc1-2协同AD5-10诱导FLS凋亡.
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| 关 键 词： | 类风湿性关节炎 成纤维样滑膜细胞 AD5-10 表阿霉素 |
Combination of AD5-10 and epirubicin in treating rhumatoid arthritis |
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| Zhou Jian-suo,Shi Juan,Zhu Jie-qing,Yuan Hai-qin,Liu Yan-xin,You Xin,Zheng De-xian. Combination of AD5-10 and epirubicin in treating rhumatoid arthritis[J]. Acta Academiae Medicinae Sinicae, 2011, 33(4): 367-370. DOI: 10.3881/j.issn.1000-503X.2011.04.004 |
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| Authors: | Zhou Jian-suo Shi Juan Zhu Jie-qing Yuan Hai-qin Liu Yan-xin You Xin Zheng De-xian |
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| Affiliation: | ZHOU Jian-suo,SHI Juan,ZHU Jie-qing,YUAN Hai-qin,LIU Yan-xin,YOU Xin,ZHENG De-xian National Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China |
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| Abstract: | Objective To investigate the mechanism of anti-death receptor 5-10(AD5-10) combined with epirubicin in treating rheumatoid arthritis(RA).Methods We detected the cell viability of the fibroblast-like synoviocytes(FLS) from RA patients with MTT.The expression level of apoptosis signaling pathways protein,p53,and p21 were evaluated with Western blot.Results We found that epirubicin,at different doses,could enhance the effect of AD5-10 on FLS,promoting the apoptosis of FLS.The expression levels of caspase-3,-8,... |
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| Keywords: | rheumatoid arthritis fibroblast-like synoviocytes AD5-10 epirubicin |
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