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| 浓缩自体骨髓复合纤维蛋白胶修复兔桡骨陈旧性骨缺损 | |
| 作者姓名： | Yao WX Ma A Zhu LL Lu K Zhu M Peng ZX Bian ZY He QF |
| 作者单位： | 1. 杭州市第一人民医院骨科,杭州,310006 2. 浙江省医学科学院寄生虫病研究所诊断室,杭州,310003 3. 上海交通大学医学院附属第九人民医院骨科,上海,200011 |
| 摘 要： | 目的 采用浓缩自体骨髓复合纤维蛋白胶修复陈旧性骨缺损,初步探讨其促进成骨的机制.方法 将36只新西兰大白兔随机平均分为3组:单纯纤维蛋白胶组(A组),纤维蛋白胶复合自体骨髓组(B组),纤维蛋白胶复合浓缩自体骨髓组(C组).每只动物均在左侧桡骨中段制造长1.5cm的段缺性骨与骨膜缺损,1个月后在骨缺损处分别植入3种方法制备的组织工程复合材料.取浓缩骨髓、纤维蛋白胶复合物行电镜观察、长期培养及细菌学培养.术后4、8、12周行X线检查、硬组织切片观察成骨效果,并行半定量分析.结果 电镜下可见骨髓有核细胞与纤维蛋白胶相容性良好,无细菌污染,长期培养无致瘤性.X线观察显示B组和C组的修复效果明显高于A组.术后8、12周,C组的Yang氏评分分别为(9.348±0.364)和(12.664±0.388)分,明显高于B组的(7.984±0.229)(F＝40.167,P＝0.001)和(10.584±0.836)分(F＝ 20.3647,P＝0.004).B组(F＝ 36.004,P＝0.001)和C组(F＝155.141,P＝0.000)术后12周的Yang氏评分均明显高于术后8周.随着时间的延长,组织学切片见B组和C组均有不同程度的骨缺损修复.结论 自体骨髓或浓缩自体骨髓复合纤维蛋白胶可修复兔桡骨陈旧性骨缺损,单纯纤维蛋白胶无此作用. |
| 关 键 词： | 纤维蛋白组织黏着剂 骨髓 骨缺损 骨代用品成骨 |
Application of enriched bone marrow compound with fibrin glue in repairing old radial bone defect in rabbits |
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| Yao WX,Ma A,Zhu LL,Lu K,Zhu M,Peng ZX,Bian ZY,He QF.Application of enriched bone marrow compound with fibrin glue in repairing old radial bone defect in rabbits[J].Acta Academiae Medicinae Sinicae,2011,33(4):387-392. | |
| Authors: | Yao Wang-xiang Ma An Zhu Liu-long Lu Kai Zhu Min Peng Zhao-xiang Bian Zhen-yu He Qi-fang |
| Institution: | YAO Wang-xiang1,MA An2,ZHU Liu-long1,LU Kai1,ZHU Min1,PENG Zhao-xiang3,BIAN Zhen-yu1,HE Qi-fang1 1Department of Orthopaedics,First Hospital of Hangzhou,Hangzhou 310006,China 2Department of Diagnosis,Institute of Parasitic Diseases,Zhejiang Academy of Medical Sciences,Hangzhou 310003,China 3Department of Orthopaedics,Shanghai Ninth people's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China |
| Abstract: | Objective To explore the feasibility of using enriched bone marrow(BM) compound with fibrin glue(FG) in repairing old radial bone defect.Methods Totally 36 New Zealand rabbits were equally randomized into three groups: simple FG group,BM+FG group,and enriched BM+FG group.A 1.5-cm segmental bone defect was made at the left radial in each animal.After one month,the defect was implanted with the engineered bone.Before implantation,a compound of enriched BM with FG underwent electron microscopy,long-term cultur... |
| Keywords: | fibrin tissue adhesive bone marrow bone defects bone substitutes |
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