灵芝醇溶酸性组分的制备工艺与检测方法研究

目的 :研究灵芝子实体中醇溶酸性组分 (ethanol solubleandacidiccomponents ,ESAC)制备工艺 ,并建立一种定量测定EASC含量的方法。方法 :灵芝精粉用无水乙醇浸泡 ,碱提酸化和氯仿萃取得ESAC组分 ;利用ESAC与浓硫酸的特征性颜色反应进行定量测定。结果 :1 0 g级ESAC较优的制备工艺为 :灵芝精粉与无水乙醇的投料比 1 (g)∶1 5(mL) ,浸提时间 24h ,饱和NaHCO₃溶液和氯仿萃取量分别为 1300mL ,分 3次萃取。ESAC定量测定条件为 :样品量 1g ,溶剂 15mL ,浸泡 0.5h ,50%H₂SO4 乙醇溶液 ,100℃水浴加热 3min ,测定 490nm处光吸收值。结论 :本文提供的ESAC制备的工艺路线 ,具有设计合理、操作简便的特点 ,建立的ESAC含量的测定方法可以用于灵芝相关产品的质量鉴定。

Study on Preparation Process and Analytical Methods of ESAC from Ganoderma lucidum

OBJECTIVE: To develop the procedure for separating the ethanol-soluble and acidic components (ESAC) from Ganoderma lucidum, and to establish a method for quantifying ESAC in G. lucidum. METHOD: The ethanol extract of G. lucidum was extracted with a saturated NaHCO3 solution, acidified and re-extracted by chloroform to obtain ESAC. The quantitative analysis of ESAC was based on the characteristic color reaction between ESAC and H2SO4. RESULT: The optimal conditions for separating ESAC on a 10 g scale are as follows: ratio of material and ethanol (mL), 1:15; immersing time, 24 h; volume of saturated NaHCO3 and chloroform, 1300 mL; extract 3 times. The condition for measuring ESAC is as follows: sample weight, 1 g; solution volume, 1.5 mL; immuersing time, 0.5 h; detecting reagent, 50% H2SO4 in ethanol; heating time in 100 degrees C water bathe, 3 min; measuring wavelength, 490 nm. CONCLUSION: The procedure for ESAC preparation is simple and well-designed, and the established method for ESAC can be used for the qualitative analysis of the G. lucidum related products.