| PI3K在CD_3mAb活化T细胞信号转导中的作用 |
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| 引用本文： | 石晓红,侯彦强,孙晓军. PI3K在CD_3mAb活化T细胞信号转导中的作用[J]. 山东医药, 2009, 49(27): 38-39 |
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| 作者姓名： | 石晓红 侯彦强 孙晓军 |
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| 作者单位： | 1. 山东省千佛山医院,济南,250014
2. 上海交通大学附属第一人民医院松江分院 |
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| 摘 要： | 目的探讨磷脂酰肌酶-3激酶（PI3K）在CD3mAb活化T细胞信号转导途径中的作用。方法分离获取健康人外周血单个核细胞（PBMC）,用不同浓度的PI3K特异性抑制剂LY294002处理后再用CD3mAb活化T细胞。0、6、12、24、48、72h后检测总T细胞CD69分子的表达及IL-2表达情况,培养10d后计数总T细胞的增殖情况。结果LY294002呈浓度依赖性地抑制总T细胞CD69的表达、IL-2的产生和T细胞增殖。结论PI3K参与CD3mAb诱导T淋巴细胞活化的信号转导途径,对T细胞的充分活化必不可少。
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| 关 键 词： | T淋巴细胞 磷脂酰肌酶-3激酶 |
Role of PI3K in the signal transduction pathway of T cells activated by CD3mAb |
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| SHI Xiao-hong,HOU Yan-qiang,SUN Xiao-jun. Role of PI3K in the signal transduction pathway of T cells activated by CD3mAb[J]. Shandong Medical Journal, 2009, 49(27): 38-39 |
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| Authors: | SHI Xiao-hong HOU Yan-qiang SUN Xiao-jun |
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| Affiliation: | SHI Xiao-hong , HOU Yan-qiang, SUN Xiao-jun (1 Shandong Qianfoshan Hospital, Jinan 250014 ,P. R. China) |
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| Abstract: | Objective To investigate the role of PI3K in the signal transduction pathway of T cells activated by CD3mAb.Methods Health human peripheral blood mononuclear cells（PBMC）were separated,and treated with different concentrations of PI3K inhibitor LY294002,then the T cells activated by CD3mAb.Expression of CD69 molecule and IL-2 in total T cells were detected 0,6,12,24,48,72 h after the treatment,the proliferation of total T cells were counted 10d after the cultivation.Results LY294002 inhibited the CD69 expression,IL-2 generation and T cell proliferation of total T cells dose-dependently.Conclusions PI3K involves in CD3mAb-induced T lymphocyte activation signal transduction pathways,and it is essential for the activation of T cells. |
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| Keywords: | T lymphocyte PI3K |
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