RP-HPLC法同时测定西洋参含片中4种成分

目的建立同时测定西洋参含片中人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1及拟人参皂苷F11含量的RP-HPLC方法。方法色谱柱为Agilent TC-C18(250 mm×4.6 mm,5μm);流动相为0.5 mL·L~(-1)磷酸溶液(A)-乙腈(B),梯度洗脱;流速:1.0mL·min~(-1);检测波长:203nm;柱温:30℃。结果人参皂苷Rg_1和人参皂苷Re及人参皂苷Rb_1在2.5~500μg·mL~(-1)、拟人参皂苷F11在5.0~1 000μg·mL~(-1)范围内线性关系良好(r≥0.999 4);平均加样回收率均大于95.0%。结论该方法简便、准确,重复性好,可作为西洋参含片的质量控制方法。

Simultaneous determination of four components in Panax quinque f olius L .Tablets by RP-HPLC

Objective To establish an RP-HPLC method for simultaneous determination of four components in Panax quinquefolius L .Tablets ,including gensenoside Rg1 ,gensenoside Re ,gensenoside Rb1 and pseuoginsenoside F11 .Methods The analysis was per-formed on an Agilent TC-C18 column (250 mm × 4 .6 mm ,5 μm) with 0 .5 mL?L-1 phosphoric acid-acetonitrile with step gradient elu-tion .The flow rate was 1 .0 mL?min-1 with detection wavelength of 203 nm .The column temperature was kept at 30 ℃ .Results The ginsenoside Rg1 ,ginsenoside Re ,ginsenoside Rb1 and pseuoginsenoside F11 showed good linear relationship in the ranges of 2 .5-500 .0 ,2 .5-500 .0 ,2 .5-500 .0 and 5 .0-1 000 .0 μg?mL-1 ,respectively .All of the average recovery rates were more than 95 .0% .Conclusion This method is simple ,accurate ,reliable and reproducible .It can be used for the quality control of Panax quinque f olius L .Tablets .