黄药子及黄药子配伍当归对大鼠肝组织grp78和bad基因表达的影响

目的：研究单用黄药子及黄药子配伍当归对大鼠肝组织grp78和bad基因表达的影响,探讨黄药子引起肝损伤以及配伍当归后其损伤减轻的机制。方法：30只清洁级SD雄性大鼠随机分为黄药子组、黄药子配伍当归组和对照组,每组10只。上述3组大鼠分别给予黄药子提取物混悬液（1mL相当于黄药子0.067g）、黄药子和当归提取物混悬液（1mL相当于黄药子0.067g,当归0.133g）及0.5%羧甲基纤维素钠（CMC）溶液各2mL,灌胃,2次/d,持续14d。末次给药24h后采用实时定量聚合酶链式反应（实时定量PCR）的方法测定各组大鼠肝脏grp78和bad基因的表达。结果：3组大鼠肝脏grp78和bad基因表达通过看家基因gapdh校正后的结果如下：黄药子组grp78和bad基因的2^-△△Ct分别为339.9和256.2,黄药子配伍当归组分别为17.0和9.9,对照组均为1.0。与对照组比较,黄药子组grp78和bad基因表达明显上调,差异有统计学意义（P〈0.01）;而与黄药子组比较黄药子配伍当归组基因表达有显著的下调趋势,差异有统计学意义（P〈0.01）。结论：黄药子可上调大鼠肝脏grp78和bad基因的表达,这可能是黄药子引起肝损伤的机制;当归对黄药子致大鼠肝脏grp78和bad基因表达上调的拮抗作用可能是其减轻黄药子所致肝损害的机制之一。

Effects of Dioscorea bulbifera rhizome and Dioscorea bulbifera rhizome plus Angelica sinensis root on expression of grp78 and bad genes in rat livers

Objective：To study the expression of grp78 and bad genes in rat livers after giving Dioscorea bulbifera rhizome alone and Dioscorea bulbifera rhizome plus Angelica sinensis root in order to explore the mechanism of Dioscorea bulbifera rhizome induced liver damage and actions of Angelica sinensis root on alleviation of liver damage induced by Dioscorea bulbifera rhizome. Methods：A total of 30 clean grade SD male rats were divided randomly into three groups：the Dioscorea bulbifera rhizome group,the Dioscorea bulbifera rhizome plus Angelica sinensis root group and the control group. Each group comprised 10 rats. The rats in the 3 groups mentioned above were respectively gavaged with 2 mL of the suspension of Dioscorea bulbifera rhizome extracts （1 mL of the suspension was equivalent to Dioscorea bulbifera rhizome 0.067g）,2 mL of the suspension of Dioscorea bulbifera rhizome extracts plus Angelica sinensis root extracts （1 mL of the suspension was equivalent to Dioscorea bulbifera rhizome 0.067 g and Angelica sinensis root 0.133 g）,and 2 mL of 0.5% sodium carboxymethyl cellulose （CMC） solution twice daily for 14 days. The expression of grp78 and bad genes in rat livers were measured by quantitative real-time PCR （real time Q-PCR） 24 hours after the last administration. Results：After it was corrected by house-keeping gene gapdh,the expression of grp78 and bad genes in rat livers in the three groups were as follows：2-△△Ct of grp78 and bad genes in the Dioscorea bulbifera rhizome group were 339.9 and 256.2,respectively; 17.0 and 9.9 in the Dioscorea bulbifera rhizome plus Angelica sinensis root group,respectively; 1.0 and 1.0 in the control group,respectively. The expression of grp78 and bad genes were up-regulated in the Dioscorea bulbifera rhizome group compared with the control group. The difference was significant（P〈0.01）. The expression of grp78 and bad genes were down-regulated in the Dioscorea bulbifera rhizome plus Angelica sinensisroot group compared with the Dioscorea bulbifera rhizome group. The difference was significant（P〈0.01）. Conclusion：Dioscorea bulbifera rhizome can increase the expression of grp78 and bad genes in rat livers,which may be the mechanism of liver damage induced by Dioscorea bulbifera rhizome. Antagonistic actions of Angelica sinensis root on the expression of Dioscorea bulbifera rhizome-induced up-regulated grp78 and bad genes in rat livers is one of the possible mechanisms of alleviation of Dioscorea bulbifera rhizome-induced liver damage.