脂脉宁对香烟提取物诱导血管内皮细胞-304的NF-KB及热休克蛋白-70表达影响

目的：观察含脂脉宁血清对香烟提取物（CSE）诱导血管内皮细胞（VEC）～304的核转录因子-xB（NF—xB）及热休克蛋白-70（HSP-70）表达的影响。方法：将VEc-304细胞分为4组：CSE组、实验1组（脂脉宁大剂量）、实验2组（脂脉宁小剂量）及空白对照组。采用免疫组化方法，观察各组VEC-304细胞NF—KB及HSP-70的表达。结果：CSE组细胞NF—KB[（288±25）：（185±14）]、HsP-70[（370±28）：（258±20）]的表达均较空白对照组明显升高[P〈O．05～O．01]，而脂脉宁两组NF—KB[1组（193±17），2组（211±14）]、HSP-70[1组（262±21），2组（282±23）]表达明显低于CSE组[P〈O．05～0．01]，且1组作用更明显（P〈0．05）。细胞DAB显色：CSE组细胞NF—xB蛋白、HsP-70蛋白显色呈棕黄或棕褐色，明显较空白对照组色深；实验1组和2组显色虽较空白对照组为深，但明显较CSE组色浅。结论：香烟提取物可诱导血管内皮细胞核转录因子-kB、热休克蛋白～70表达．脂脉宁则可使其表达下调。

Effects of Zhimaining on expressions of nuclear factor--KB and heat shock protein--70 in vascular endothelial cells--304 induced by cigarette smoke extract

Objective： To investigate the effects of Zhimaining in serum on the expressions of nuclear factor kB （NF-- kB） and heat shock protein--70 （HSP--70） in vascular endothelial cells-304 （VEC-304） induced by cigarette smoke extract （CSE）. Methods： The expressions of NF-- kB and HSP--70 in four cell groups： CSE group, group 1 （treated with high dose of Zhimaining）, group 2 （treated with low dose of Zhimaining） and normal control group were test by immunohistoehemical method. Results： The expressions of NF--kB E （288±25） vs. （185±14）1 and HSP--70 E （370 ±28） vs. （258±20）] in CSE group were significantly higher than those of normal control group [P〈0. 05-0.01], and the expressions of NF-kB rgroupl （193±17）, group2 （211±14）] and lISP--70 [groupl （262± 21）, group2 （282±23）] in the two groups treated with Zhimaining were significantly lower than those of CSE group ]P〈0. 05- 0.01], and it＇s more obvious in group 1. For cell DAB coloration test, NF--kB and HSP- 70 showed brown--yellow or brown in CSE group, and they were significantly darker than those of normal control group. Though the colors in group1, 2 were darker than those of normal control group, but significantly lighter than those of CSE group （P〈 0.05）. Conclusion： Cigarette smoke extract could up--regulate the expressions of nuclear factor--KB and heat shock protein--70 in vascular endothelial cells--304, whereas, Zhimaining could down-regulate them.