Genotypic subtyping of hepatitis C virus |
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| Authors: | KAZUAKI CHAYAMA AKIHITO TSUBOTA YASUJI ARASE SATOSHI SAITOH ISAO KOIDA KENJI IKEDA TOYOMI MATSUMOTO MARIKO KOBAYASHI SATOMI IWASAKI SHIMA KOYAMA TSUTO MORINAGA HIROMITSU KUMADA |
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| Affiliation: | Department of Gastroenterology, TEIJIN Ltd, Hino-Shi, Japan;*Liver Research Laboratory, Toranomon Hospital, Tokyo and TEIJIN Ltd, Hino-Shi, Japan;†Department of Molecular Biology, TEIJIN Ltd, Hino-Shi, Japan |
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| Abstract: | Four subtypes of hepatitis C virus (HCV), Pt(I), K1(II), K2a(III) and K2b(IV), have been suggested based on the nucleotide sequences of the non-structural (NS) 5 region. A fifth subtype from Japanese patients, Tr(V), which shows a less than 68% homology in nucleotide sequence when compared with other subtypes has been identified. A one-step method which enables a quick determination of subtype using polymerase chain reaction with a mixed primer set deduced from the sequence of each subtype has been developed. Using this technique, the subtypes of 418 out of 478 Japanese patients (87.4%) were determined. The incidence of each subtype in Japan was as follows: K1(II), 307 (73.4%); K2a(III), 74 (17.7%); K2b(IV), 28 (6.7%); and Tr(V), 3 (0.7%). This one-step subtyping technique should be useful for studying the epidemiology or biology of the HCV. |
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| Keywords: | mixed primer set nucleotide sequences polymerase chain reaction |
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