三氯乙烯致敏豚鼠体内循环免疫复合物的变化

目的检测循环免疫复合物(CIC)在三氯乙烯(TCE)致敏豚鼠体内含量的变化,探讨其可能的作用。方法选用体重250～300 g的白色雌性豚鼠,随机分成空白对照组、溶剂对照组、TCE处理组。根据豚鼠最大值试验(GPMT)方法处理豚鼠。分别在实验结束后24 h、72 h采血,检测血清中IgA/C3-CIC、IgG/C3-CIC和IgM/C3-CIC的浓度。结果 TCE处理组致敏率为65.38%;与溶剂对照组相比,TCE致敏组和TCE未致敏组的血清中IgA/C3-CIC、IgG/C3-CIC和IgM/C3-CIC的浓度均明显降低,差异有统计学意义(P<0.05)。结论 TCE处理组豚鼠血清中循环免疫复合物含量降低,可能提示其体液免疫发生改变。

Changes of circulating immune complexes in guinea pigs sensitized by trichloroethylene

Objective To explore possible role of circulating immune complexes(CIC)played in guinea pigs(GPs)sensitized by trichloroethylene(TCE)and the related mechanism.Methods White female GPs(250～300 g)were randomly divided into blank control group,solvent(olive oil)control group and TCE treated group.GPs were treated according to Guinea Pig Maximization Test(GPMT).24 h and 72 h after administration,the blood samples were taken for detection of serum levels of IgA/C3-CIC,IgG/C3-CIC and IgM/C3-CIC with Kits.Results Sensitization rate of GPs by TCE was 65.38%,compared with those of solvent control group,the levels of IgA/C3-CIC,IgG/C3-CIC and IgM/C3-CIC in unsensitized and sensitized PGs by TCE were all significantly decreased(P<0.05).Conclusion The study suggested that TCE treatment may affect the humoral immunity in GPs.