miR-125b、miR-29a和miR-155-5p作为诊断结核性脑膜炎生物标志的研究

目的 探讨结核性脑膜炎患者脑脊液及血浆中 microRNA (miR)-125b、 miR-29a 和 miR-155-5p 的表达水平及其临床意义。方法 收集 20 例结核性脑膜炎患者（结脑组）和 20 例原发性头痛患者（对照组）的脑脊液和血浆， 分别提取总 RNA， 使用实时定量 PCR 方法分别检测脑脊液和血浆中 miR-125b、 miR-29a 及 miR-155-5p 的表达水平。结果 与对照组相比， 结脑组脑脊液、 血浆中 miR-29a、 miR-125b 表达水平明显上调， 结脑组血浆中 miR-155-5p 表达水平明显上调， 差异均有统计学意义 （均 P<0.01）， 而 2 组脑脊液中 miR-155-5p 表达水平比较差异无统计学意义。结论 miR-125b、 miR-29a 和 miR-155-5p 可能参与了调节结核性脑膜炎的发生、 发展过程， miR-125b和 miR-29a有可能作为潜在的诊断结核性脑膜炎的生物学标志。

Study of miR-125b,miR-29a and miR-155-5p as biomarkers of tuberculous meningitis

Objective To investigate the expression levels of microRNA(miR)-125b, miR-29a and miR-155-5p in cerebrospinal fluid (CSF) and plasma from the patients with tuberculous meningitis and their clinic significance. Methods Cerebrospinal fluid and plasma samples were collected from 20 patients with tuberculous meningitis (tuberculous meningitis group) and 20 patients suffered from primary headache (control group). The total RNAs were extracted.The levels of miR- 125b, miR-29a and miR-155-5p were determined by real-time quantitative PCR (q-PCR). Results Levels of miR-29a and miR-125b in both CSF and plasma of patients in tuberculous meningitis group were significantly higher than those in patients from control group with statistical significance (P<0.01). Mean time, the level of miR-155-5p in plasma but not in CSF of patients in tuberculous meningitis group was higher than those in control group with statistical significance (P<0.01). Conclusion Expression of miR-125b, miR-29a and miR-155-5p may participate in regulating the occurrence and development of tuberculous meningitis. And miR-125b and miR-29a may be used as potential biomarkers for diagnosing tuberculous meningitis.