| 牛蒡ITS 序列与药材质量的相关性研究 |
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| 引用本文： | 许亮,窦德强,王冰,杨燕云,康廷国. 牛蒡ITS 序列与药材质量的相关性研究[J]. 中国中药杂志, 2011, 36(14): 1931-1935 |
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| 作者姓名： | 许亮 窦德强 王冰 杨燕云 康廷国 |
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| 作者单位： | 辽宁中医药大学 药学院,辽宁大连,116600 |
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| 基金项目： | 国家"十一五"科技支撑计划项目(2006BAIO09B02-6);辽宁省教育厅课题(2009A496);辽宁中医药大学青年药学人才基金项目(YXRC0920) |
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| 摘 要： | 目的 研究牛蒡ITS序列与牛蒡子药材质量相关性.方法 采集全国26个不同产地的牛蒡子药材,采用PCR扩增后测定ITS序列,HPLC法对牛蒡子药材中牛蒡苷含量进行测定,应用ClustaiX( 1.81),Mage 4.0,SPSS 13.0等统计软件进行遗传多样性、基因分型、相关性等分析.结果 测得了26份牛蒡的ITS序列全长,在GenBank中注册,获得登记号,测定牛蒡子药材中牛蒡苷含量、千粒重,统计分析牛蒡的基因分型与药材质量具有相关性.结论 为揭示牛蒡子道地药材的分子形成机制奠定了基础.
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| 关 键 词： | 牛蒡 ITS序列 药材质量 |
| 收稿时间： | 2011-03-05 |
Study on correlation between ITS sequence of Arctium lappa and quality of Fructus Arctii |
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| XU Liang,DOU Deqiang,WANG Bing,YANG Yanyun and KANG Tingguo. Study on correlation between ITS sequence of Arctium lappa and quality of Fructus Arctii[J]. China Journal of Chinese Materia Medica, 2011, 36(14): 1931-1935 |
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| Authors: | XU Liang DOU Deqiang WANG Bing YANG Yanyun KANG Tingguo |
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| Affiliation: | Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;Liaoning University of Traditional Chinese Medicine, Dalian 116600, China |
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| Abstract: | Objective: To study the correlation between ITS sequence of Arctium lappa and Fructus Arctii quality of different origin. Method: The samples of Fructu arctii materials were collected from 26 different producing areas. Their ITS sequence were determined after polymerase chain reaction (PCR) and quality were evaluated through the determination of arctiin content by HPLC. Genetic diversity, genotype and correlation were analyzed by ClustalX(1.81),Mage 4.0,SPSS 13.0 statistical software. Result: ITS sequence of A.was obtained from 26 samples, and was registered in the GenBank. Corresponding arctiin content of Fructus arctii and 1 000-grain weight were determined. A. lappa genotype correlated with Fructus arctii quality by statistical analysis. Conclusion: The research provided a foundation for revealing the molecular mechanism of Fructus arctii geoherbs. |
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| Keywords: | Arctium lappa ITS sequence Fructus Arctii quality |
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