丹皮酚脂质体体外透皮试验

目的制备丹皮酚脂质体,考察其作为经皮给药载体的透皮特性。方法薄膜-超声法制备丹皮酚脂质体;采用透皮扩散仪,以小鼠皮肤进行体外经皮渗透实验,考察丹皮酚脂质体的经皮渗透行为及皮内滞留药物量。结果制得的丹皮酚脂质体主要为单室脂质体,平均包封率为72.8%,平均粒径为163.8 nm。体外经皮渗透试验表明,与50%的乙醇溶液相比,脂质体透皮速率较慢,但皮肤中药物滞留量明显增加。结论丹皮酚脂质体制备工艺可行,能使药物在皮肤中蓄积,持续平稳释药。

Study on transdermal permeation of paeonol liposomes in vitro

Objective To prepare the paeonol liposomes and investigate its penetration characteristics as a transdermal vehicle.Methods The liposomes were prepared by a film-ultrasonic technique.We conducted the examination of in vitro transdermal permeation on rat's skin with diffusion device to evaluated the transdermal permeation ability and the drug amount left in the skin.Results The liposomes prepared were mono-layer vehicles with an average entrapment rate of 72.8% and the particle size of 163.8 nm.Compared with the solution of 30% ethanol,the liposomes had lower transdermal speed but higher drug content in the skin.Conclusions The preparation method of paeonol liposomes was proved to be applicable.The paeonol liposomes can make the drug accumulate in skin,which was expected to release steadily and continuously.