鼻息肉中肥大细胞雌激素受体的表达及其意义

目的 检测雌激素受体在鼻息肉肥大细胞和ＣＤ４＾＋、ＣＤ８＾＋及ＣＤ６８＾＋细胞上的表达。方法 采用抗肥大细胞，抗ＣＤ４＾＋、ＣＤ８＾＋、ＣＤ６８＾＋细胞以及抗雌激素受体的单顾隆抗体对９例鼻息肉标本进行免疫组化及双标免疫染色。结果 所有上述细胞亚群中相应抗体清晰地检测到。形态学观察，雌激素受体阳性细胞明显与ＣＤ４＾＋、ＣＤ８＾＋、ＣＤ６８＾＋细胞不同，但也肥大细胞相似，双标免疫洒以，只有肥大细胞而非

Expression of estradiol receptors in mast cells of the upper airway]

OBJECTIVE: To examine the expression of estrogen receptors in mast cells, CD4+, CD8+ and CD68+ cells in human nasal polyps. METHODS: Immunohistochemical and dual immunostaining methods were used with mouse monoclonal antibodies against human estrogen receptors, a range of human immune cell types included CD4+ (helper T-cells), CD8+ (suppressor/cytotoxic T-cells), CD68+ (Macrophages) cells and mast cells. RESULTS: Coincident with the known features of human nasal polyps, all the immune/inflammatory cell types examined were clearly detected with corresponding monoclonal antibodies. The cells positive for estrogen receptor were morphologically similar to the mast cells but distinct from CD4+, CD8+ or CD68+ cells. The cells stained for estrogen receptors by dual immunostaining coincided exactly with cells labeled as mast cells but differed from CD4+, CD8+ or CD68+ cells. CONCLUSION: Estrogen receptors express only in mast cells, but not in CD4+, CD8+ or CD68+ cells in the human upper airway.