| 高效液相色谱法测定理中汤配方颗粒中人参皂苷含量 |
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| 引用本文： | 刘俊达,胡昌江,李兴华. 高效液相色谱法测定理中汤配方颗粒中人参皂苷含量[J]. 中国药业, 2007, 16(15): 22-23 |
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| 作者姓名： | 刘俊达 胡昌江 李兴华 |
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| 作者单位： | 1. 成都理工大学材料与化学化工学院,四川,成都,610059
2. 成都中医药大学药学院,四川,成都,610075 |
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| 摘 要： | 目的 建立理中汤配方颗粒中人参皂苷的含量测定方法.方法 采用高效液相色谱（HPLC）法,以Diamonsil-C18柱（150 mm×4.6 mm,5 μm）为分析柱,流动相为乙腈-0.05%磷酸溶液（99∶400）,检测波长为203 nm,流速为1.00 mL/min,柱温为25℃.结果 进样量线性范围人参皂苷Rg1为0.992～6.944 μg（r=0.999 9）,人参皂苷Re为0.819 2～5.734 4 μg（r=0.999 8）.人参皂苷Rg1平均回收率为97.24%,RSD为1.33%（n=9）;人参皂苷Re平均回收率为97.21%,RSD为1.16%（n=9）.结论 该方法可靠、准确、简便.
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| 关 键 词： | 理中汤配方颗粒 人参皂苷 高效液相色谱法 含量测定 |
| 文章编号： | 1006-4931（2007）05-0022-02 |
| 修稿时间： | 2007-05-10 |
Determination of Ginsenoside in Lizhongtang Compatible Granule by HPLC |
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| Liu Junda,Hu Changjiang,Li Xinghua. Determination of Ginsenoside in Lizhongtang Compatible Granule by HPLC[J]. China Pharmaceuticals, 2007, 16(15): 22-23 |
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| Authors: | Liu Junda Hu Changjiang Li Xinghua |
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| Affiliation: | 1. College of Materials and Chemistry and Chemical Engineering, Chengdu University of Technology, Chengdu, Sichuan, China 610059; 2. College of Pharmacy, Chengdu University of TCM, Chengdu, Sichuan, China 610075 |
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| Abstract: | Objective To establish the determination method of ginsenoside content in Lizhongtang compatible granule. Methods The Diamonsil - C18 column （ 150 mm × 4.6 mm, 5 μm） was used. The mobile phase was acetonitrile - 0.05% phosphoric acid （99 ： 400） and the detecting wavelength was at 203 nm. The flow rate was 1.00 mL/min and the column temperature was 25℃. Results The linear range of ginsenoside Rg1 content determination was 0. 992- 6. 944 μg, r = 0. 999 9. The linear range of ginsenoside Ro content determination was 0. 819 2-5.734 4 μg, r =0. 999 8. The average recovery rate of ginsenoside Rgl was 97.24%, RSD = 1.33% （n = 9）. The average recovery rate of mnsenoside R4 was 97.21%. RSD = 1.16% （ n = 9）. Conclusion The method is reliable, accurate and simple. |
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| Keywords: | Lizhongtang compatible granule ginsenoside HPLC content determination |
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