原头节抗原IFAT和IEST诊断人体包虫病的比较研究

以冰冻保存的绵羊棘球蚴内原头节为抗原作间接荧光抗体试验(IFAT)和间接免疫酶染色试验(IEST),检测对包虫病人、非包虫病人及键康人的敏感性、特异性及交叉反应性,并与间接血凝试验相比较(IHAT)。结果表明IFAT的敏感性和特异性分别为90.36％和97.67％;IEST的敏感性和特异性分别为96.55％和100.0％;两种方法均高于IHAT的敏感性(85.54％)和特异性(95.35％)。三种方法对血吸虫感染者和癌症病人血清均无交叉反应,但对囊虫病人的交叉反应率为IFAT(51.35％)>IHAT(37.84％)>IEST(21.62％)。以原头节为抗原IEST诊断人体包虫病敏感性高、特异性强,适用范围广,不需特殊设备,具有现场应用价值。

A COMPARISON OF INDIRECT FLUORESCENT ANTIBODY TEST AND IMMUNOENZYMATIC STAINING TECHNIQUE WITH PROTOSCOLEX ANTIGENS IN THE DIAGNOSIS OF HUMAN HYDATIDOSIS

This article deals with the diagnostic values of IFAT and IEST in human hydatidosis with protoscoleces from sheep liver Echtnococcus granulosus cysts as antigens and, the results are compared with those of IHAT with cyst fluid as antigen. Positive rates for IFAT, IEST and IHAT were 90.36%(75/83), 96.55% (84/87) and 85.54% (71/83) and, false positive rates for the 3 methods were 2.33% (2/86) ,0% (0/82) and 4.65% (4/85) , respectively. No cross-reaction was observed by the 3 methods in subjects with Schistosoma japonicum infection and carcinoma,while cross-reaction rates observed in patients with cysticeri infec- tion were 51.35% by IFAT, 37.84% by IHAT and 21.62% by IEST. The above results indicate that IEST with protoscolex antigen is a more economical and effective method of higher sensitivity and specificity in the diagnosis of human hydatidosis.