L-精氨酸和一氧化氮对早期糖尿病大鼠肾小球高灌注的影响

采用链脲佐菌素（ＳＴＺ）诱导的大鼠糖尿病模型，分别在糖尿病形成后７天、１４天观察肾脏情况以及血浆和组织匀浆中一氧化氮代谢产物ＮＯ－３的水平变化与Ｌ－精氨酸（Ｌ－Ａｒｇ）的关系。结果：在不同时期肾重与体重之比明显增加（Ｐ＜０．０５），且在病变第７天即出现大量蛋白尿，在第１４天增加更明显（Ｐ＜０．０００１）；肌酐清除率（Ｃｃｒ）在不同时期也明显增高（Ｐ＜０．０５）；血浆及组织匀浆中的ＮＯ－３随时间延长有升高趋势，Ｌ－Ａｒｇ灌注后的ＮＯ－３升高显著（Ｐ＜０．０５），提示糖尿病早期即有肾脏病变，而Ｃｃｒ的升高则间接表明了肾小球滤过率升高。据此可以认为ＮＯ及Ｌ－Ａｒｇ可能是影响肾小球高滤过高灌注的重要的介导性介质。

L arginine and Nitric Oxide Have Effects on Glomerulus Hyperperfusion of Early Diabetic Rats

We used streptozotocin (STZ) to induce the animal model of diabetes mellitus in rats. On the 7th and 14th days of induction, kidney disorder, the levels of NO - 3 in plasma and tissue homogenate in different periods were observed. The NO - 3 levels in relation to the application of L arginine (L Arg) were also noted. The results showed that the kidney weight/body weight ratio significantly increased in different periods ( P <0.05). On the 7th day profuse proteinuria appeared and it markedly increased on the 14th day; creatinine clearance rate (Ccr) significantly raised, too ( P <0.005). The NO - 3 levels in plasma and tissue homogenate were getting higher with time. The level of NO - 3 significantly increased after L Arg was perfused. It indicated that kidney disorder was present at the early stage of diabetes, and the raised Ccr indirectly indicated the increase of glomerular filtration rate. These suggest that NO - 3 and L Arg may be important mediums which have effects of hyperfiltration and hyperperfusion of glomerulus.