小鼠子宫内膜细胞的原代培养与鉴定

目的:建立小鼠围着床期子宫内膜细胞的原代培养,并对其进行形态学鉴定。方法:通过挤压法获取小鼠子宫内膜组织,胶原酶Ⅰ消化,过滤法,进行单层培养。通过倒置显微镜观察其大体形态,免疫细胞化学染色法对间质细胞(ESC)及上皮细胞(EEC)进行鉴定。结果:EEC和ESC经角蛋白抗体和波形蛋白抗体染色,胞质被染成棕黄色的细胞为阳性细胞。结论:成功培养了原代小鼠子宫内膜细胞,方法简便有效,获得细胞成活率高,生长稳定。成功建立子宫内膜细胞原代体外培养体系,为从细胞和分子水平方面研究子宫内膜的各种功能及干预调节奠定基础。

Primary Culture and Its Assessment of Mice Endometrial Cell

Objective:To establish primary culture of mice endometrial cells during the embryo implantation stage,and to assess the morphology of the cultured cells.Methods:Mice endometrial cells were obtained through extrusion,digested with type Ⅰ collagenase,and subsequently cultured via monolayer cell culture after filtration.The morphology of stained endometrial epithelial cells(EEC) and endometrial stromal cells(ESC) via Immunohistochemistry were then observed under an inverted phase contrast microscope.Results:After staining with Cytokeratin-18 antibody and Vimentin antibody,the kytoplasm of positive EEC and ESE cells exhibit a brownish yellow color.Conclusion:We have successfully grown mice endometrial cells via a protocol that is simple and effective.The cultured cells have a high survival rate and show stable growth.The establishment of primary cell culture of mice endometrial cells will help further investigation of the functions and their regulations of endometrial cells at the cellular and molecular levels.