膀胱癌组织中癌基因ras和C－erbB－2蛋白产物的免疫组织化学研究

采用免疫组织化学ＡＢＣ方法检测５６例膀胱癌组织中的癌基因ｒａｓ和Ｃ－ｅｒｂＢ－２蛋白产物，有３０例和１９例分别呈ｒａｓ和Ｃ－ｅｒｂＢ－２蛋白产物表达阳性，ｒａｓ表达在分化较高、表浅性和非复发性肿瘤中高于分化较差、浸润性和复发性者，而Ｃ－ｅｒｂＢ－２蛋白表达在高分级、浸润性和复发性肿瘤中较高。ｒａｓ和Ｃ－ｅｒｂＢ－２蛋白产物表达与膀胱癌组织分级、临床分期和复发密切相关。

Immunohistochemical Evaluation of the ras and C-erbB-2 Oncogene Protein Product in Bladder Carcinoma

Expression of the ras and the C-erbB-2 oncogene products was investigated in 56 cases of human bladder transitional cell carcinoma using an immunohistochemical method. Thirty of the 56 cases of bladder tumor were found to be immunohistologically positive with the anti-ras protein antibody, while 19 of 56 cases were positive with the anti-C-erbB-2 oncoprotein antibody. The ras oncoprotein positive staining was found more frequently in the well differentiated, superficial and non-recurrent tumors than in the poorly differentiated, invasive and recurrent tumors, while the C-erbB-2 gene product was more commonly detected in high-grade, invasive and recurrent tumors.Thus, the expression of either ras or C-erbB-2 was closely associated with the histological grade,clinical stage and recurrence of bladder carcinomas.