以双合成肽为抗原建立的ELISA试验在鼻咽癌血清学诊断中的应用价值 |
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引用本文: | 朱欢欢,张哲,谢莹,韩琳,Jaap M.Middeldorp,唐朝贤,黄光武,温文胜.以双合成肽为抗原建立的ELISA试验在鼻咽癌血清学诊断中的应用价值[J].中国医学文摘:肿瘤学,2011(1):52-55. |
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作者姓名: | 朱欢欢 张哲 谢莹 韩琳 Jaap M.Middeldorp 唐朝贤 黄光武 温文胜 |
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作者单位: | [1]广西医科大学第一附属医院耳鼻咽喉头颈外科,南宁530021 [2]广西医科大学实验中心,南宁530021 [3]Department of Pathology,Vrije Universiteit Medical Center, Amsterdam Netherlands |
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基金项目: | 广西自然科学基金资助项目(桂科自0728120) |
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摘 要: | 目的研究EBNA1和VCA-P18双多肽包被(EBNA1+VCA-P18)的ELISA检测在鼻咽癌(NPC)血清学诊断中的价值。方法用合成肽EBNA1和VCA-P18建立ELISA法,检测37例病理确诊的鼻咽癌患者血清和33例健康人血清,并与国产EBVVCA/IgAELISA试剂盒比较。结果双多肽EBNA1+VCA-P18/IgA灵敏度为91.9%,明显高于国产试剂盒的83.8%;二者特异度相同;EBNA1+VCA-P18/IgA的阳性预测值、阴性预测值、阳性似然比及优势比均高于国产试剂盒。结论双多肽EBNA1+VCA-P18/IgA相比商品化试剂盒在鼻咽癌血清学检测中有更高的灵敏度,适用于鼻咽癌早期诊断和人群筛查。
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关 键 词: | 鼻咽癌 血清学检查 EBNA1 VCA-P18 ELISA |
The value of double peptide-coated ELISA test in serological diagnosis of nasopharyngeal carcinoma |
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Institution: | ZHU Huan-huan,ZHANG Zhe,XIE Ying,et al.(1The First Affiliated Hospital of Guangxi Medical University,2 Experimental Center of Guangxi Medical University,Nanning 530021,China.3Department of Pathology,Vrije Universiteit Medical Center,Amsterdam Netherlands) |
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Abstract: | ObjectiveTo study the value of double peptides EBNA1 and VCA-P18-coated ELISA kit (EBNA1+VCA-P18/IgA) in serological diagnosis of nasopharyngeal carcinoma (NPC).Methods Using synthetic peptides EBNA1 and VCA-P18 to establish ELISA assay,then to detect the serum samples from 37 cases of pathologically confirmed NPC and 33 healthy controls.The commercial ELISA kit EBV VCA-P18/IgA was used as comparison.Results The sensitivity of kit EBNA1+VCA-P18/IgA was 91.9%,which was higher than that of the kit EBV VCA-P18/IgA (83.8%).Both kits had same specificity.All of the positive predictive value,negative predictive value,positive likelihood ratio and odds ratio of kit EBNA1+VCA-P18/IgA were higher than of kit VCA-P18/IgA.Conclusions Compared to commercial kit for serological detection of NPC,double peptide-coated kit EBNA1+VCA-P18/IgA is more sensitive,and it is applicable in early diagnosis and screening of NPC. |
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Keywords: | Nasopharyngeal carcinoma Serological diagnosis EBNA1 VCA-P18 ELISA |
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