比较基因组杂交技术检测肝细胞癌染色体异常及其临床意义

目的探讨肝细胞癌染色体异常及其临床意义。方法运用比较基因组杂交技术检测25例肝细胞癌染色体DNA异常情况，并与临床指标作相关分析。结果25例肝癌均存在不同程度的染色体DNA拷贝数的扩增或缺失，较为常见的染色体DNA异常是＋1q（72％）、＋1p（64％）、＋2q（、48％）、＋2p（48％）、＋5q（48％）、＋Xq（48％）、＋7q（44％）、-4q（48％）、-16p（48％）、-8p（40％）、-17p（36％）；相关分析显示＋17p、＋18p、-8p、-13q、-11q、-8q染色体异常事件与临床指标部分相关。结论肝细胞癌存在明显的染色体异常，部分染色体异常事件是非随机性的，可能与肝癌的发生、发展有关，并与肿瘤的生物学行为和预后相关。

Clinical signification of chromosomal aberration in hepatocellular carcinoma determined by comparative genomic hybridization

Objective To screen chromosomal aberration in hepatocellular carcinoma （HCC） and to explore the clinical signification. Methods Chromosomal aberration was detected by comparative genomie hybridization （CGH） in 25 patients with HCC, and the correlation between chromosomal aberration and clinical parameters were analyzed. Results All patients presented certain aberrations in copy number of chromosomal DNA. The amplification of copy number was commonly detected in chromo- some 1q （72. 0% ） ,lp （64. 0% ） ,2q （48. % ） ,2q （48. 0% ） ,Sq （48.0%） and 7q （44. 0% ） ,while the loss of copy number was often found in chromosome 4q （48. 0% ） ,16p （48.0%） ,8p （40. 0% ） and 17p （36. 0% ）. The amplification of 17p and 18p as well as the loss of 8p, 13q, 11 q and 8q showed significant correlation with some clinical parameters. Conclusions Chro- mosomal aberrations occur frequently in HCC. Some of the nonrandom chromosomal alterations may relate to the development, biological behavior and prognosis of HCC.