WFDC-1基因在原发性肝癌组织中的表达及其临床意义

目的检测WFDC-1基因在肝癌和正常肝组织的表达差异并探讨其临床意义。方法采用荧光实时定量PCR（FQ-RT-PCR）法检测30例肝癌组织及相应远端正常肝组织WFDC-1mRNA的表达,分析其表达与肝癌患者临床特征的相关性。结果 WFDC-1mRNA在肝癌组织中的表达水平（0.050±0.026）明显低于远端正常肝组织（0.113±0.040）,两者差别有统计学意义（P〈0.05）。WFDC-1mRNA在肝癌中的表达水平与HBsAg水平有关,合并HBsAg阳性的肝细胞癌（HCC）中的表达低于HBsAg阴性HCC组（P〈0.05）,而与年龄、性别、肿瘤大小、临床分期、AFP及有无肝硬化无关（P〉0.05）。结论肝癌组织中WFDC-1基因表达水平明显低于相应正常肝组织,提示WFDC-1表达与降低肿瘤发生有一定的相关性,为潜在的抑癌基因。

Expression of WFDC-1 in primary hepatocellular carcinoma and its clinical significance

Objective To explore the expression of WFDC-I in hepatocellular carcinoma （HCC） and its clinical signifi- cance. Methods By fluorescent quantitative real time polymerase chain reaction （FQ-RT-PCR）, the expression of WFDC-1 mRNA were detected in 30 cases of HCC tissues, the corresponding surrounding liver tissues of which were used as controls. Results The expression level of WFDC-1 mRNA in HCC tissues （0. 050 ±0. 026） was lower than that of the corresponding surrounding liver tissue （0. 113 ± 0. 040） ,which wasm was significantly different （ P 〈 0. 05 ）. The expression of WFDC-1 mRNA in HCC tissues was closely correlated with the HBV infection marker HBsAg： the expression level of WFDC-1 mRNA was significantly lower in HBsAg-positive HCC tissues than that of HBsAg-negative HCC tissues （ P 〈 0.05 ）. The expression of WFDC-1 mRNA was not significantly correlated with patient＇ s clinicopathological characters such as age, sex, stage, tumor size, etc （P 〉 0. 05）. Conclusions The expression level of WFDC-1 mRNA in HCC tissues was significantly lower than that in the corresponding surrounding liver tissue. WFDC-1 gene may be a new potential suppressor gene for HCC.