人参皂苷Rg3联合三氧化二砷抑制乳腺癌移植瘤生长及其新生血管形成的研究

目的探讨人参皂苷Rg3（简称Rg3）联合三氧化二砷（As2O3）对裸鼠人乳腺癌移植瘤模型中肿瘤生长及新生血管密度的影响。方法右前肢腋窝皮下移植人乳腺癌MCF-7细胞株的雌性裸鼠28只,随机分成4组：①联合用药组即Rg3（12mg/kg.d）＋As2O3（45μg/kg.d）;②Rg3组（12mg/kg.d）;③三氧化二砷组（45μg/kg.d）;④对照组（0.5m l/d）。自肿瘤接种第5天开始,Rg3隔日灌胃,共25次;三氧化二砷腹腔内注射,每日1次,共35次,期间观察裸鼠的生存质量。停药1周后,脱颈处死裸鼠,检测肿瘤的重量,并计数肿瘤内微血管密度（MVD）。结果 Rg3组、三氧化二砷组、联合用药组对裸鼠人乳腺癌移植肿瘤生长的抑制作用明显,联合用药组抑制肿瘤生长的作用比单一用药组更强,联合用药组MVD明显低于三氧化二砷组及对照组。结论 Rg3与三氧化二砷联合应用具有协同作用,可明显抑制乳腺癌新生血管形成,降低乳腺癌组织内的MVD,进而抑制裸鼠乳腺癌移植瘤的生长,同时降低三氧化二砷的毒副反应。

Inhibitory effect of ginsenoside Rg3 combined with arsenic trioxide on tumor growth and neoangiogenesis in transplanted breast carcinoma

Objective To study the effect of ginsenoside Rg3（Rg3） combined with arsenic trioxide（As2O3） on reducing the microvessel density（MVD） in transplanted breast carcinoma of nude mice.Methods 28 female nude mice were divided randomly into 4 groups,received Rg3（12mg/kg,qd） plus As2O3（45μg/kg,qd）,Rg3（12mg/kg·d） alone,As2O3（45μg/kg,qd） alone or Saline（0.5 ml,qd）,respectively.Five days after transplantation,Rg3 was given to the mice by gastrogavage once every 2 days for total 25 times;As2O3 was injected intraperitoneally once a day for 35 days.One week after finishing the treatment,all the animals were killed and the sizes of solid tumors were measured.The MVD within the tumors was examined by immunohistochemical staining.Results The tumor weight of the three treated groups was significantly lower than that of control group.The tumor weight in Rg3 plus As2O3 group was lower than that in Rg3 group and As2O3 group.The MVD value in Rg3 plus As2O3 group was significantly lower than that of As2O3 group and control group.Conclusion Rg3 combined with As2O3 has synergistic effect which can inhibit angiogenesis of malignant tumor,reduce MVD within tumor,inhibit growth of the transplanted breast carcinoma in nude mice and reduce toxicity of arsenic trioxide.