重症肌无力病人血清非免疫球蛋白成分中的14KD蛋白

在21例抗体阳性、20例抗体阴性重症肌无力(MG),18例其他神经科疾病(OND)患者和19例正常对照血清中制备非免疫球蛋白(Ig)成分。并予以聚丙烯酰胺凝胶电泳(SDS-PAGE)和等电聚焦(IEF)电泳分析。结果显示,大多数MG病人的非Ig成分电泳图谱中有一分子量为14KD的特异蛋白区带,其出现频度以抗体阴性MG(85％)最高,与抗体阳性MG(52.38％)比较差异有显著性(P<0.05)。14KD蛋白可能参与了MG的发病,尤其在抗体阴性MG中的作用可能更为重要。

A 14KD Protein of the Non immunoglobulin Serum Components in My asthenia Gravis

By salt precipitation, immune affinity absorption and Protein G Fast Flow, the non-Ig components were prepared from the serum of 21 seropositive myasthenia gravis (MG) patients, 20 seronegative MG patients, 18 patients with other neurological diseases (OND) and 19 health controls. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing electrophoresis (IEF) were employed to analyse the non-Ig components of MG, OND and normal controls. A specific 14KD molecule weight protein was found in the majority of the electrophoresis patterns of the non-Ig components of MG. The frequency of the 14KD protein is the highest in the seronegative MG (85%), with significant difference from that (52. 38%) of seropositive MG (P<0. 05). We suppose that the 14KD protein might participate in the pathogenesis of MG, and might play a more important role in the seronegative MG.