人血清高密度脂蛋白磷含量的测定

目的 建立人血清高密度脂蛋白磷酯（HDL－PL）的测定方法。方法 采用磷钨酸及氯化镁沉淀法将血清中含载脂蛋白B脂蛋白沉淀去除，上清液用于HDL－PL测定。结果 只需2．5－3．0分钟时间就能将HDL－磷脂完全消化。血清HDL－PL测定结果的变异系数为3．6％－3．7％，回收率为98％－107％，平均回收率为103％。对30例正常人，30例Ⅱa型高脂蛋白症、30例Ⅳ型高脂蛋白血症及30例Ⅱb型高脂蛋白血症患者血清HDL－PL及HDL－C进行了测定，发现Ⅳ型高脂蛋白血症患者血清HDL－PL显著低于正常人和Ⅱa型高脂蛋白血症患者，其它各组之间血清HDL－PL无显著性差异。而HDL－C在正常人及Ⅱa型高脂蛋白血症患者与Ⅱb型高脂血症患者及Ⅳ型高脂血症患者间比较均有显著性差异。结论 人HDL－PL代谢与体内甘油三酯代谢的关系更为密切，HDL－PL降低可能是检测Ⅳ型高脂蛋白血症的更好的指标。

Establishment and implication of an assay for high density lipoprotein phospholipids in human serum]

OBJECTIVE: To develop an assay for high density lipoprotein phospholipids in human serum based on ascorbutate reduction method. METHODS: HDLs were separated from apolipoprotein B-containing lipoproteins by precipitation of phosphotungstic acid and magnesium chloride. Phospholipids of HDL were extracted by ethanol/ether, and dried. After the dried phospholipids were digested by sulphuric acid and perchloric acid, the color was developed by adding ammonium molybdate in ascorbutate. The levels of high density lipoprotein phospholipids (HDL-PL) were measured by spectrophotometry at 700 nm. RESULTS: The coefficients of variation (CV) were 3.6% and 3.7% within two batches of assays. Recovery of isolated HDL-PL added to serum ranged from 98% to 107%, averagely 103%. The established assay for human serum HDL-PL was used to measure the serum levels of 30 hypercholesterolemic subjects, 30 hypertriglyceridemic subjects, 30 combined hyperlipidemic subjects, and 30 normolipidemic subjects. The hypertriglyceridemic subjects had lower HDL-PL level than normolipidemic subjects and hypercholesterolemic subjects (The P values are 0.005 and 0.007 respectively). CONCLUSION: A simple and specific method for assay of HDL-phos-pholipids in human serum has been developed. The above data collected by the use of this method demonstrate the closer relationship between human HDL-PL metabolism and triglyceride metabolism, suggesting that lower HDL-PL level might serve as an index in the assay for type IV hyperlipidemia.