气腹对家兔肺损伤作用的初步研究

目的探讨家兔气腹模型中不同气腹持续时间对肺的损伤作用。方法以家兔为研究对象随机分为对照组（N组,假气腹组）、短时间气腹组（S组,气腹时间小于1h）、长时间气腹组（L组,气腹时间大于3h）。取各组血浆测血浆蛋白含量,分别取肺组织称取湿干重计算肺组织含水量、测定髓过氧化物酶（myleopemxidase,MPO）含量,取支气管肺泡灌洗液（bronchoalveolar lavage fluid,BALF）离心后测定上清液蛋白含量和沉渣中白细胞,计算肺通透系数（lung permeability index,LPI）。结果 L组肺组织含水量及BALF中白细胞数大于S和N组（P〈0.01）,肺组织MPO活力明显高于S和N组。结论大于3h的持续气腹对肺组织造成了损伤,这种损伤,可能与中性粒细胞在肺内的募集、引发炎症反应等机制有关。

The Effect of Pneumoperitoneum induced Lung Injury in Rabbits

Objective T o evaluat e the effect of pneumoperitoneum induced lung injury in rabbit. Methods 30 rabbits were randomly assigned to following groups. The control group（ N） was subjected to a sham pneumoperitoneum. The short time group（ S） was subjected to 1 hour of pneumoperitoneum. The long time group （ L） was subjected to 3 hours of pneumoperitoneum. After 20 min of desufflation subsequent to cessation of pneumoperitoneum,blood,lung,and bronchoalveolar lavage fluid（ BALF） samples were obtained from every rabbit of the groups. white blood cell（ WBC） count in BALF were detennined. The myleopemxidase（ MPO） activity and wet to dry weight ratio（ W/D） were also measured. Results Lung permeability index（ LPI） and WBC count in BALF of group（ L） are higher than the other t wo groups（ P 0. 01） ,The lung tissue MPO activity of group（ L） is much higher than group（ S） and group（ N） （ P 0. 01） . Conclusion Lung injury induced by more than 3 hours of pneumoperitoneum is more effective than 1 hour of pneumoperitoneum. PMNs in lung tissue maybe responsible for the inflammatory response induced by more than 3 hours of pneumoperitoneum rabbit lungs.